Structural Biology

Lectures

Unit 1

1. Introduction: Why to Study Structural Biology Video
2. Introduction to Biological Macromolecules Video
3. Introduction: Decoding Biological Macromolecules Video
4. Introduction: Genome Sequencing Video
5. Introduction: Post Genomic Era Video

Unit 2

6. Amino acids and their properties Video
7. Protein: Protein Chemistry, Chirality, Peptide bond and Levels of protein structures Video
8. Protein: Dihedral angles, Peptide bond and Ramachandran Plot Video
9. Protein: Super Secondary Structures, Motif, Domains, Non-covalent interactions Video
10. Protein: Folding of Protein, Thermodynamics and Kinetics of protein folding, Characterization of Proteins Video

Unit 3

11. Introduction to Structural Biology Techniques Part I Video
12. Introduction to Structural Biology Techniques Part II Video
13. X-ray Crystallography: Crystallization Part I Video
14. X-ray Crystallography: Crystallization Part II Video
15. X-ray Crystallography: Crystal Mounting Video

Unit 4

16. X-ray Crystallography: Production of X-ray and its properties Video
17. X-ray Crystallography: Journey to 3D land Video
18. X-ray Crystallography: Crystal Symmetry Video
19. X-ray Crystallography: Instrumentation in X-ray Crystallography Video
20. X-ray Crystallography: Data collection and processing Video

Unit 5

21. X-ray Crystallography: Data Analysis Part I Video
22. X-ray Crystallography: Data Analysis Part II Video
23. X-ray Crystallography: Phase Problem Part I Video
24. X-ray Crystallography: Phase Problem Part II Video
25. X-ray Crystallography: Refinement and Structure deposition to PDB Video

Unit 6

26. Introduction to Spectroscopy and NMR Video
27. Basic Principles of NMR and Instrumentation Video
28. NMR Sample Preparation and Chemical Shift related concepts Video
29. Factors effecting NMR Spectra (1D & 2D) Video
30. 2D & 3D NMR Spectroscopy focusing on protein structure Video

Unit 7

31. Introduction to Spectroscopy. Video
32. UV-Vis and CD spectroscopy Video
33. Fluorescence Spectroscopy and Green Fluorescence Protein (GFP) Video
34. Infrared & Raman Spectroscopy for protein Video
35. Raman Spectroscopy, Raman Microscopy and Raman Crystallography for studying protein Video

Unit 8

36. Introduction to Microscopy Video
37. Functioning details of Cryo Electron Microscopy (Cryo EM) Video
38. Cryo Electron Microscopy: Data Collection and Analysis Video
39. A concise story of advancement Cryo-EM Video
40. Protein Data Bank Video

Unit 9

41. History of Molecular Visualizations of Biological Macromolecules Video
42. Description of structure related files (.pdb, .mmcif, .mtz, etc.) Video
43. Demonstration of COOT Video
44. 3D visualization using Pymol Video
45. Demonstration of Pymol Video

Unit 10

46. Why we need MD Simulation Video
47. Molecular Dynamic Simulation Process: Part I Video
48. Molecular Dynamic Simulation Process: Part II Video
49. Molecular Dynamic Simulation Process: Part III Video
50. Application of Molecular Dynamic Simulation Video

Unit 11

51. What, How & Which of Protein Engineering Video
52. How to make logical Protein Engineering: Process of Rational design Video
53. Success story of Rational Protein designing: Focusing on De Novo Process Video
54. Designing Protein by mimicking nature: Process of Directed Evolution Video
55. Achievement, Challenges, and Future direction in the field of Protein Engineering Video

Unit 12

56. Introduction to Structure Based Drug Discovery (SBDD) Video
57. Rational Drug Discovery Video
58. Docking Based Virtual Screening: Progress, Challenges and Future perspective Video
59. What makes a small molecule an ideal drug: Developing in silico ADMETox Model Video
60. Structure Based Drug Discovery: Case study and Conclusion Video