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Course10210655336 lectures

Advanced Fluorescence Microscopy and Image Processing

Prof. Gunjan Mehta · IIT Hyderabad

Start with lecture 1 At NPTEL

Lectures

week-01

  1. Lecture 01_Introduction to Fluorescence Microscopy Video
  2. Lecture 02_Introduction to Optics Video

week-02

  1. Lecture 03_Design of a fluorescence microscope Video
  2. Lecture 04_Fluorescent proteins, organic dyes, and protein labelling strategies Video
  3. LIVE _Demonstration of the fluorescence microscope, its design and components Video

week-03

  1. Lecture 05_6D imaging, live cell imaging, time lapse imaging Video
  2. Lecture 06_Quantifying protein dynamics using FRAP, FLIP, FRET Video
  3. LIVE_Demonstration for 6D imaging of live cells on a Leica DMi8 inverted fluorescence microscope Video

week-04

  1. Lecture 07_Types of Illumination Epi, TIRF, HILO, light sheet, two photon Video
  2. Lecture 08_Confocal microscopy Video
  3. LIVE_Demonstration of the Nikon N-SPARC confocal microscope Video

week-05

  1. Lecture 09_Super-Resolution Microscopy (SIM, STED, STORM/PALM) Video
  2. Lecture 10_Nanomaterials for fluorescence imaging Video

week-06

  1. Lecture 11_Immunofluorescence IF and Immunohistochemistry IHC Video
  2. Lecture 12_Fluorescence In Situ Hybridization FISH, RNA FISH Video
  3. LIVE_Demonstration for immunofluroescence procedure Video

week-07

  1. Lecture 13_Digital images and camera technologies for microscopy Video
  2. Lecture 14_CCD, EMCCD, sCMOS Camera Video
  3. LIVE_Demonstration for digital images and adjustments Video

week-08

  1. Lecture 15_Basic operations of ImageJ/FIJI, montage preparation Video

week-09

  1. Lecture 16_Image filtering and object segmentation Video

week-10

  1. Lecture 17_Macros, intensity measurement, scale bar, time stamp, colocalization Video
  2. LIVE_Demonstration of ImageJ/Fiji based image processing Video

week-11

  1. Lecture 18_Single molecule imaging and tracking Video
  2. Lecture 19_Optical tweezers and traction force microscopy Video
  3. LIVE_Demonstration for single-molecule imaging under HILO illumination Video

week-12

  1. Lecture 20_Spatial transcriptomics and proteomics RNAscope, MERFISH, CODEX Video
  2. Lecture 21_High content imaging Video
  3. Lecture 22_Designing a fluorescence microscopy experiment Video

Live Session

  1. Live Session 31-01-2025 Video
  2. Live Session 07-02-2025 Video
  3. Live Session 12-02-2025 Video
  4. Live Session 28-02-2025 Video
  5. Live Session 07-03-2025 Video
  6. Live Session 28-03-2025 Video
  7. Live Session 11-04-2025 Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Hyderabad
Department
Biotechnology
Teacher
Prof. Gunjan Mehta
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
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