Learn · Advanced Fluorescence Microscopy and Image Processing

Lecture 1 of 36week-01

Lecture 01_Introduction to Fluorescence Microscopy

Prof. Gunjan Mehta · IIT Hyderabad

Credits and licence

From
Advanced Fluorescence Microscopy and Image Processing, IIT Hyderabad (at NPTEL)
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
Who can watch
Tweens profiles and older. How courses are placed

All the lectures

  1. Lecture 01_Introduction to Fluorescence Microscopy Video
  2. Lecture 02_Introduction to Optics Video
  3. Lecture 03_Design of a fluorescence microscope Video
  4. Lecture 04_Fluorescent proteins, organic dyes, and protein labelling strategies Video
  5. LIVE _Demonstration of the fluorescence microscope, its design and components Video
  6. Lecture 05_6D imaging, live cell imaging, time lapse imaging Video
  7. Lecture 06_Quantifying protein dynamics using FRAP, FLIP, FRET Video
  8. LIVE_Demonstration for 6D imaging of live cells on a Leica DMi8 inverted fluorescence microscope Video
  9. Lecture 07_Types of Illumination Epi, TIRF, HILO, light sheet, two photon Video
  10. Lecture 08_Confocal microscopy Video
  11. LIVE_Demonstration of the Nikon N-SPARC confocal microscope Video
  12. Lecture 09_Super-Resolution Microscopy (SIM, STED, STORM/PALM) Video
  13. Lecture 10_Nanomaterials for fluorescence imaging Video
  14. Lecture 11_Immunofluorescence IF and Immunohistochemistry IHC Video
  15. Lecture 12_Fluorescence In Situ Hybridization FISH, RNA FISH Video
  16. LIVE_Demonstration for immunofluroescence procedure Video
  17. Lecture 13_Digital images and camera technologies for microscopy Video
  18. Lecture 14_CCD, EMCCD, sCMOS Camera Video
  19. LIVE_Demonstration for digital images and adjustments Video
  20. Lecture 15_Basic operations of ImageJ/FIJI, montage preparation Video
  21. Lecture 16_Image filtering and object segmentation Video
  22. Lecture 17_Macros, intensity measurement, scale bar, time stamp, colocalization Video
  23. LIVE_Demonstration of ImageJ/Fiji based image processing Video
  24. Lecture 18_Single molecule imaging and tracking Video
  25. Lecture 19_Optical tweezers and traction force microscopy Video
  26. LIVE_Demonstration for single-molecule imaging under HILO illumination Video
  27. Lecture 20_Spatial transcriptomics and proteomics RNAscope, MERFISH, CODEX Video
  28. Lecture 21_High content imaging Video
  29. Lecture 22_Designing a fluorescence microscopy experiment Video
  30. Live Session 31-01-2025 Video
  31. Live Session 07-02-2025 Video
  32. Live Session 12-02-2025 Video
  33. Live Session 28-02-2025 Video
  34. Live Session 07-03-2025 Video
  35. Live Session 28-03-2025 Video
  36. Live Session 11-04-2025 Video