Course10210609360 lectures
Cellular Biophysics: a Framework for Quantitative Biology
Prof. Chaitanya A.Athale · IISER Pune
About
Given than most biological systems are in fact out of equilibrium, this course will touch upon some of the most recent theoretical and experimental approaches to understand the out of equilibrium aspects of biophysics. To this end we make a thermodynamics detour to answer the question posed by Schroedinger: “What is life?” We then consider a diversity of cell types to ask how the hierarchies of molecules and cells integrate. The question of how stochastic processes lead to deterministic outcomes will be briefly touched upon. Research paper reading will highlight case studies of the successful application of physics biological problems.
INTENDED-AUDIENCE: 3rd year B.Sc, 1-2 year M.Sc. students in Biology and Physics
Prerequisites: BSc/BE/BTech 2nd year; BSc level knowledge of Classical mechanics; MSc level knowledge of Cell and molecular biology; MSc level knowledge of Biochemistry; Basic python programmingINDUSTRY-SUPPORT: Microscopy companies, Centrifuge companies, Spectroscopy companies
Lectures
Week 1
- Introduction - Part 01
- Introduction - Part 02
- Introduction - Part 03
- Solids vs Fluids
- Viscosity
- Measuring Viscosity
- Tutorial - Part 01
- Tutorial - Part 02
- Tutorial - Part 03
Week 2
- Water: Macromolecular Nature & Hydrophobicity, Structure of Ice, Pauling-Bernal-Fowler Model of Water
- Entropy and Probability of Water Conformations, Boltzmann Law of Entropy
- Reynolds Number
- Tutorial - Part 01
- Tutorial - Part 02
- Tutorial - Part 03
- Hagen-Poisuielle Equation
- Tutorial - Part 04
Week 3
- Sedimentation and Centrifugation - Part 1
- Sedimentation and Centrifugation - Part 2
- Blood Centrifugation
- Review: Paperfuge for Hematology
- Biology by Numbers
- Biology by Numbers: Bomb Yield Solved
- Order of Magnitude Estimates and Approximations
- Physical Basis of Life
- Approximating Cellular and Molecular Size Scales
Week 4
- Quantifying DNA and Chromatin
- Protein Abundance and Spacing
- Model Gene
- Cell-Biology by Numbers
- Experimental Techniques to Quantify Cells
- Time-Scales in Cells
- Energy Scale
- Energy and Thermodynamics of Life - Part: 01
Week 5
- Energy and Thermodynamics of Life - Part: 02
- Energy and Life- Osmotic Engine
- Energy and Life- Interconversion of Energy
- Random Walk Statistics, Stoke-Einstein - Part 01
- Random Walk Statistics, Stoke-Einstein - Part 02
- Demonstration of Diffusion of Micron Sized Particles
Week 6
- Macromolecular Crowding - Part 01
- Macromolecular Crowding - Part 02
- Cytoskeleton
- Beam Theory Applied to Biopolymer
- Understanding Chromosomes as Statistical Polymers - Part 01
- Understanding Chromosomes as Statistical Polymers - Part 02
Week 7
- Brownian Ratchets and Molecular Motors
- Polymerization Dynamics - Part 1
- Polymerization Dynamics - Part 2
- Polymerization Dynamics - Part 3
- Python Programming - Part 1
- Python Programming - Part 2
- Python Programming - Part 3
Week 8
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IISER Pune
- Department
- Biotechnology
- Teacher
- Prof. Chaitanya A.Athale
- Level
- Graduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
- Who can watch
- NPTEL gives no rating. Invisible Network shows it to Teens profiles and older, judged from what the source says about it. How courses are placed
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