Learn · Basics of Fluroscence Spectroscopy
Lecture 39 of 40Week 8 - Single Molecule Spectroscopy
Lecture 39: Single molecule detection (Cont.)
Prof. Pratik Sen · IIT Kanpur
Credits and licence
- From
- Basics of Fluroscence Spectroscopy, IIT Kanpur (at NPTEL)
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- Tweens profiles and older. How courses are placed
All the lectures
- Lecture 01: Absorption of light and fluorescence
- Lecture 02: Absorption of light and molecular energy levels
- Lecture 03: Electronic transition and FrankCondon principle
- Lecture 04: Measurement of Absorption spectrum
- Lecture 05: Solvent effect on absorption spectrum
- Lecture 06: ET(30) polarity scale
- Lecture 07: Fate of excited state and Jablonski diagram
- Lecture 08: Properties and parameters of fluorescence
- Lecture 09: Fluorimeter
- Lecture 10: Fluorescence spectrum measurement
- Lecture 11: Corrected emission spectrum
- Lecture 12: Fluorescence quantum yield
- Lecture 13: Solvent effect on fluorescence
- Lecture 14: Solvent effect on fluorescence (Cont.)
- Lecture 15: LippertMataga equation
- Lecture 16: Solvation dynamics and fluorescence lifetime
- Lecture 17: TCSPC
- Lecture 18: TCSPC (Cont.)
- Lecture 19: TCSPC (Cont.)
- Lecture 20: TCSPC (Cont.)
- Lecture 21: Fluorescence UpConversion
- Lecture 22: Fluorescence Quenching
- Lecture 23: Combined static and dynamic quenching
- Lecture 24: Fractional quenching
- Lecture 25: Application of fluorescence quenching
- Lecture 26: Fluorescence anisotropy
- Lecture 27: Fluorescence anisotropy (Cont.)
- Lecture 28: Timeresolved fluorescence anisotropy
- Lecture 29:Magic angle and Perrin equation
- Lecture 30: Initial anisotropy
- Lecture 31:Energy transfer
- Lecture 32:FRET
- Lecture 33:FRET (Cont.)
- Lecture 34:Excited state proton transfer
- Lecture 35:Excited state proton transfer (Cont.)
- Lecture 36:Excimer and Exciplex
- Lecture 37:Single molecule fluorescence
- Lecture 38: Single molecule detection
- Lecture 39: Single molecule detection (Cont.)
- Lecture 40: Fluorescence correlation spectroscopy