Learn · NPTEL

Course10410408440 lectures

Basics of Fluroscence Spectroscopy

Prof. Pratik Sen · IIT Kanpur

Start with lecture 1 At NPTEL

Lectures

Week 1 - Introduction to Fluorescence

  1. Lecture 01: Absorption of light and fluorescence Video
  2. Lecture 02: Absorption of light and molecular energy levels Video
  3. Lecture 03: Electronic transition and FrankCondon principle Video
  4. Lecture 04: Measurement of Absorption spectrum Video
  5. Lecture 05: Solvent effect on absorption spectrum Video

Week 2 - Instrumentation for Fluorescence Spectroscopy

  1. Lecture 06: ET(30) polarity scale Video
  2. Lecture 07: Fate of excited state and Jablonski diagram Video
  3. Lecture 08: Properties and parameters of fluorescence Video
  4. Lecture 09: Fluorimeter Video
  5. Lecture 10: Fluorescence spectrum measurement Video

Week 3 - Solvent and Environmental Effects

  1. Lecture 11: Corrected emission spectrum Video
  2. Lecture 12: Fluorescence quantum yield Video
  3. Lecture 13: Solvent effect on fluorescence Video
  4. Lecture 14: Solvent effect on fluorescence (Cont.) Video
  5. Lecture 15: LippertMataga equation Video

Week 4 - Time-Domain Lifetime Measurements

  1. Lecture 16: Solvation dynamics and fluorescence lifetime Video
  2. Lecture 17: TCSPC Video
  3. Lecture 18: TCSPC (Cont.) Video
  4. Lecture 19: TCSPC (Cont.) Video
  5. Lecture 20: TCSPC (Cont.) Video

Week 5 - Fluorescence Quenching

  1. Lecture 21: Fluorescence UpConversion Video
  2. Lecture 22: Fluorescence Quenching Video
  3. Lecture 23: Combined static and dynamic quenching Video
  4. Lecture 24: Fractional quenching Video
  5. Lecture 25: Application of fluorescence quenching Video

Week 6 - Fluorescence Anisotropy

  1. Lecture 26: Fluorescence anisotropy Video
  2. Lecture 27: Fluorescence anisotropy (Cont.) Video
  3. Lecture 28: Timeresolved fluorescence anisotropy Video
  4. Lecture 29:Magic angle and Perrin equation Video
  5. Lecture 30: Initial anisotropy Video

Week 7 - Excited state phenomena: Energy Transfer and Proton Transfer

  1. Lecture 31:Energy transfer Video
  2. Lecture 32:FRET Video
  3. Lecture 33:FRET (Cont.) Video
  4. Lecture 34:Excited state proton transfer Video
  5. Lecture 35:Excited state proton transfer (Cont.) Video

Week 8 - Single Molecule Spectroscopy

  1. Lecture 36:Excimer and Exciplex Video
  2. Lecture 37:Single molecule fluorescence Video
  3. Lecture 38: Single molecule detection Video
  4. Lecture 39: Single molecule detection (Cont.) Video
  5. Lecture 40: Fluorescence correlation spectroscopy Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Kanpur
Department
Chemistry and Biochemistry
Teacher
Prof. Pratik Sen
Level
Undergraduate
Language
English
Subjects
Chemistry
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
Who can watch
NPTEL gives no rating. Invisible Network shows it to Tweens profiles and older, judged from what the source says about it. How courses are placed