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Lecture 12 of 42Week 2

Lecture 12: Practical Considerations: Spectral bandwidth, wavelength error & stray light

Prof. Ashutosh Kumar · IIT Bombay

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Biomolecular Spectroscopy, IIT Bombay (at NPTEL)
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All the lectures

  1. Lecture 1: Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - I Video
  2. Lecture 2: Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - II Video
  3. Lecture 3: Jablonski diagram - I Video
  4. Lecture 4: Jablonski diagram - II Video
  5. Lecture 5: Instrumentation, Beer's law and the extinction coefficient & Chromophore: factors affecting the absorption properties of a chromophore Video
  6. Lecture 6: Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium- I Video
  7. Lecture 7: Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium- II Video
  8. Lecture 8: Spectral shifts in rhodopsin and vision Video
  9. Lecture 9: Structural studies DNA using absorption spectroscopy. Helix coil transition of double stranded DNA Video
  10. Lecture 10: Melting curve of DNA: Hyperchromicity & hypochromicity Video
  11. Lecture 11: Structural studies of proteins using absorption spectroscopy Video
  12. Lecture 12: Practical Considerations: Spectral bandwidth, wavelength error & stray light Video
  13. Lecture 13: Structural studies of proteins using absorption spectroscopy Video
  14. Lecture 14: Theory of fluorescence spectroscopy- I Video
  15. Lecture 15: Photoluminescence, emission, and absorption. Stokes Shift Video
  16. Lecture 16: Theory of fluorescence spectroscopy- II Video
  17. Lecture 17: Advanced Application of Flourescence Spectroscopy Video
  18. Lecture 18: Solvent and Environmental Effects on Fluorescence Video
  19. Lecture 19: Inner Filter Effect in Fluorescence Video
  20. Lecture 20: Flourescence Lifetime Video
  21. Lecture 21: Fluorescence resonance energy transfer Video
  22. Lecture 22: Introduction to InfraRed Spectroscopy Video
  23. Lecture 23: Introduction to Vibrational Spectroscopy: Infrared Spectroscopy Video
  24. Lecture 24: Fundamerntal of FT-IR Video
  25. Lecture 25: Introduction to Raman Spectroscopy Video
  26. Lecture 26: Fundamental and Application of Raman Spectroscopy Video
  27. Lecture 27: Biological application of FT-IR Video
  28. Lecture 28: Introduction. Linearly and circularly polarized light Video
  29. Lecture 29: Instrumentation of Circular Dichroism (CD) Spectroscopy Video
  30. Lecture 30: Experimental: sample concentration and cell pathlength. Solvent effects Video
  31. Lecture 31: Characteristics of CD & ORD (optical rotatory dispersion) spectroscopy: Cotton Effect Video
  32. Lecture 32: Identification of known optical isomers Video
  33. Lecture 33: Determination of protein secondary structure Video
  34. Lecture 34: Detection of molten globule like structure of proteins Video
  35. Lecture 35: Applications of CD spectroscopy in studying amyloid based neurodegenerative disorders Video
  36. Lecture 36: Determination of nucleic acid conformations Video
  37. Lecture 37: Use of CD spectroscopy to study stacking orientation of the base pairs Video
  38. Lecture 38: Application of Spectroscopy in Biopharma Setting I Video
  39. Lecture 39: Application of Spectroscopy in Biopharma Setting II Video
  40. Lecture 40: Application of Raman Spectroscopy in Pharma and Biopharma Industry I Video
  41. Lecture 41: Application of Raman Spectroscopy in Pharma and Biopharma Industry II Video
  42. Lecture 42: Fluorescence Spectroscopy Application for various Pharmaceutical products Video