Biomolecular Spectroscopy

Lectures

Week 1

1. Lecture 1: Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - I Video
2. Lecture 2: Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - II Video
3. Lecture 3: Jablonski diagram - I Video
4. Lecture 4: Jablonski diagram - II Video
5. Lecture 5: Instrumentation, Beer's law and the extinction coefficient & Chromophore: factors affecting the absorption properties of a chromophore Video
6. Lecture 6: Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium- I Video
7. Lecture 7: Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium- II Video

Week 2

8. Lecture 8: Spectral shifts in rhodopsin and vision Video
9. Lecture 9: Structural studies DNA using absorption spectroscopy. Helix coil transition of double stranded DNA Video
10. Lecture 10: Melting curve of DNA: Hyperchromicity & hypochromicity Video
11. Lecture 11: Structural studies of proteins using absorption spectroscopy Video
12. Lecture 12: Practical Considerations: Spectral bandwidth, wavelength error & stray light Video

Week 3

13. Lecture 13: Structural studies of proteins using absorption spectroscopy Video
14. Lecture 14: Theory of fluorescence spectroscopy- I Video
15. Lecture 15: Photoluminescence, emission, and absorption. Stokes Shift Video
16. Lecture 16: Theory of fluorescence spectroscopy- II Video
17. Lecture 17: Advanced Application of Flourescence Spectroscopy Video

Week 4

18. Lecture 18: Solvent and Environmental Effects on Fluorescence Video
19. Lecture 19: Inner Filter Effect in Fluorescence Video
20. Lecture 20: Flourescence Lifetime Video
21. Lecture 21: Fluorescence resonance energy transfer Video
22. Lecture 22: Introduction to InfraRed Spectroscopy Video

Week 5

23. Lecture 23: Introduction to Vibrational Spectroscopy: Infrared Spectroscopy Video
24. Lecture 24: Fundamerntal of FT-IR Video
25. Lecture 25: Introduction to Raman Spectroscopy Video
26. Lecture 26: Fundamental and Application of Raman Spectroscopy Video
27. Lecture 27: Biological application of FT-IR Video

Week 6

28. Lecture 28: Introduction. Linearly and circularly polarized light Video
29. Lecture 29: Instrumentation of Circular Dichroism (CD) Spectroscopy Video
30. Lecture 30: Experimental: sample concentration and cell pathlength. Solvent effects Video
31. Lecture 31: Characteristics of CD & ORD (optical rotatory dispersion) spectroscopy: Cotton Effect Video
32. Lecture 32: Identification of known optical isomers Video

Week 7

33. Lecture 33: Determination of protein secondary structure Video
34. Lecture 34: Detection of molten globule like structure of proteins Video
35. Lecture 35: Applications of CD spectroscopy in studying amyloid based neurodegenerative disorders Video
36. Lecture 36: Determination of nucleic acid conformations Video
37. Lecture 37: Use of CD spectroscopy to study stacking orientation of the base pairs Video

Week 8

38. Lecture 38: Application of Spectroscopy in Biopharma Setting I Video
39. Lecture 39: Application of Spectroscopy in Biopharma Setting II Video
40. Lecture 40: Application of Raman Spectroscopy in Pharma and Biopharma Industry I Video
41. Lecture 41: Application of Raman Spectroscopy in Pharma and Biopharma Industry II Video
42. Lecture 42: Fluorescence Spectroscopy Application for various Pharmaceutical products Video