Learn · Computational Genomics
Lecture 16 of 53week-05
Estimating the Amount of Sequencing Coverage for a Genome and Hybrid Sequencing Approaches
Prof. Vineet Kumar Sharma · IISER Bhopal
Credits and licence
- From
- Computational Genomics, IISER Bhopal (at NPTEL)
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
- Who can watch
- Teens profiles and older. How courses are placed
All the lectures
- Introduction to Different OMICS Approaches and their Applications
- Genetic Information in Prokaryotic
- Databases and Web Resources to Store and Access the Biological Data
- First and Second Generation Sequencing Technologies
- Long Read Sequencing and Linked Read Sequencing - Part 01
- Long Read Sequencing and Linked Read Sequencing - Part 02
- Sequence Formats and Databases for Genomic Analysis
- Introduction to Linux
- File Handling and Remote Connectivity in Linux
- Running Linux Commands and Installation of Genomic Packages
- Introduction to R and Applications in Genomic Analysis
- Publicly Available Tools and Need for Workstations for Genomic Analysis
- Overview of Genomic and Transcriptomic Analysis
- Genomic and Transcriptomic Analysis of an Organism with Case Studies
- How to Collect & Confirm Sample of the Species to be Sequenced & Transcriptome Sequencing Approaches
- Estimating the Amount of Sequencing Coverage for a Genome and Hybrid Sequencing Approaches
- Types of Reads, Quality Filtering, Estimating the Genome Complexity and Heterozygosity
- Genome Assembly and its Completion Status, Assembly Algorithms
- Commonly Used Assembly Tools
- Linked-Read Sequencing and Processing
- Long Reads Analysis and Assembly Workflow
- De novo Assembly Using Genomic and Transcriptomic Reads
- Merging Assemblies to Create Hybrid Assembly and Determining the Quality of Assembly
- Chromosomal Level Assembly and Case Studies
- Identification and Annotation of Repeats in Genomes
- De novo Transcriptome Assembly and Making the Coding Gene Set
- Prediction of tRNA, rRNA and miRNA in a Genome
- Functional Annotation and Identification of Metabolic Pathways in a Genome
- Comprehensive Functional Annotation of Predicted Genes in a Genome
- Functional Annotation of Predicted Genes by Alternate Methods
- Methods and Steps to Perform the Evolutionary Analysis of a Genome
- Methods for Taxonomic Classification and Phylogeny Econstruction and Analysis
- Epigenetics, ChIp-seq, Transcriptome and Microarrays for Regulation of Expression
- Single Cell Genomics, 10X Chromium Linked-reads and Illumina Sequencing, Single Cell Gene Expression
- Application of Multiomics Approaches in Human Health and Diseases Such as Cancer, Diabetes, etc.
- Prokaryotic Genome Sequencing and Assembly Approaches
- Gene Prediction Approaches and Common Methods for Bacterial Gene Prediction
- Common Methods for Annotation of a Bacterial Genome, t-RNA, rRNA, Operon Prediction and Annotation
- Phylogenetic Analysis of Bacterial Genomes
- Metabolic and Comparative Analysis
- Microbiome and Metagenome, Human, Organismal and Environmental Microbiomes
- Sequencing and Assembly of Metagenomes, Gene Prediction, Annotation, MAGs (Part 1)
- Sequencing and Assembly of Metagenomes, Gene Prediction, Annotation, MAGs (Part 2)
- Taxonomic Analysis Using Amplicon Sequence Variants, Statistical Analysis
- Live Session 10-02-2024
- Live Session 17-02-2024
- Live Session 24-02-2024
- Live Session 02-03-2024
- Live Session 09-03-2024
- Live Session 16-03-2024
- Live Session 30-03-2024
- Live Session 06-04-2024
- Live Session 13-04-2024