Effects-of-Nanostructures-and-Mouse-Embryonic-Stem-Cells-on-In-Vitro-Morphogenesis-of-Rat-pone.0060054.s002

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Time lapse video sequence revealing the process of cord formation in rat testicular cells. Primary rat (7-day-old) Sertoli cells and peritubular cells were grown at an initial density of 106 cells/cm2 on nanostructured PDMS (nanogratings of 350 nm width) in DMEM. At two weeks cultures consisted of confluent monolayers with some Sertoli cell clusters. Time lapse imaging was started when first cellular movements were observed at day 14. The observation was continued for 24 hours. We recorded 720 image frames in total and converted these into a movie sequence of 144 seconds with 5 frames per second. One second in the time lapse video is equal to 10 minutes real time.

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