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Course10410510253 lectures

Experimental Biochemistry

Prof. Swagata Dasgupta and Prof. Soumya De · IIT Kharagpur

Start with lecture 1 At NPTEL

Lectures

WEEK 1

  1. Lecture 1 : Introduction Video
  2. Lecture 2 : Buffers Video
  3. Lecture 3 Introduction to Biochemistry Laboratory Equipments and Safety Measures Video
  4. Lecture 4 Practical Aspects of Making Buffer Video
  5. Lecture 5 Making Tris Buffer (pH=8.2) Video
  6. Lecture 6 Making Phosphate Buffer (100mM) Video

WEEK 2

  1. Lecture 07 : Amino Acids and Their Properties Video
  2. Lecture 08 : Amino Acid Titrations Video
  3. Lecture 09 : pI Determination of Glycine Video
  4. Lecture 10 : pI Determination of Lysine Video
  5. Lecture 11 : Summary Video

WEEK 3

  1. Lecture 12 : UV and Visible Spectroscopy Video
  2. Lecture 13 : Fluorescence Spectroscopy Video
  3. Lecture 14 : UV/Visible Spectra of Amino Acids and Proteins Video
  4. Lecture 15 : Fluorescence Spectra of Amino Acids and proteins Video
  5. Lecture 16 : Spectroscopic Techniques Summary Video

WEEK 4

  1. Lecture 17 : Protein Folding and Denaturation - I Video
  2. Lecture 18 : Protein Folding and Denaturation - II Video
  3. Lecture 19 : Urea denaturation of HSA studied by UV/Vis absorbance Video
  4. Lecture 20 : Temperature denaturation of HSA studied by UV/Vis absorbance Video
  5. Lecture 21 : Denaturation of HSA by GdnHCl studied by Trp fluorescence Video
  6. Lecture 22 : Protein Folding and Denaturation Summary Video

WEEK 5

  1. Lecture 23 : Chromatographic Techniques - I Video
  2. Lecture 24 : Chromatographic Techniques - II Video
  3. Lecture 25 : Protein Purification by Size Exclusion Chromatography (SEC) Video
  4. Lecture 26 : Protein Purification by Affinity Chromatography Video

WEEK 6

  1. Lecture 27: Gel Electrophoresis of DNA and Proteins - Part I Video
  2. Lecture 28: Gel Electrophoresis of DNA and Proteins - Part II Video
  3. Lecture 29: Gel Electrophoresis of DNA and Proteins - Part II Video

WEEK 7

  1. Lecture 30 : Isolation and Characterization of Proteins Part - I Video
  2. Lecture 31 : Isolation and Characterization of Proteins Part - II Video
  3. Lecture 32 : Isolation and Purification of Proteins Video
  4. Lecture 33 : Quality and Quantity of the Isolated Protein Video

WEEK 8

  1. Lecture 34 : Enzyme Kinetics I Video
  2. Lecture 35 : Enzyme Kinetics II Video
  3. Lecture 36 : Enzyme Kinetics (by using enzyme from apple juice) Video
  4. Lecture 37 : Enzyme Kinetics (by using enzyme from apple juice) (Contd.) Video

WEEK 9

  1. Lecture 38 : Isolation and Characterization of DNA Part - I Video
  2. Lecture 39 : Isolation and Characterization of DNA Part - II Video
  3. Lecture 40 : Bacterial Culture for Plasmid DNA Isolation Video
  4. Lecture 41 : Isolation of Plasmid DNA Video
  5. Lecture 42 : Isolation and Characterization of DNA Summary Video

WEEK 10

  1. Lecture 43 : Basics of rDNA Technology Part - I Video
  2. Lecture 44 : Basics of rDNA Technology Part - II Video
  3. Lecture 45 : Cloning : Polymerase Chain Reaction, Restriction Enzyme Digestion and Ligation Video
  4. Lecture 46 : DNA Transformation Video

WEEK 11

  1. Lecture 47 : Protein-Ligand Interaction Video
  2. Lecture 48 : Protein-Ligand Interaction (Contd.) Video
  3. Lecture 49 : Interaction study of HSA protein with Curcumin and Gallic acid using UV-Vis spectroscopy Video
  4. Lecture 50 : Interaction study of HSA protein with Curcumin and Gallic acid using UV-Vis spectroscopy (Contd.) Video
  5. Lecture 51 : Analysis of the structure of protein ligand complex Video

WEEK 12

  1. Lecture 52: Immunoassay Techniques Video
  2. Lecture 53: Western Blotting Technique Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Kharagpur
Department
Chemistry and Biochemistry
Taught by
Prof. Swagata Dasgupta, Prof. Soumya De
Level
Undergraduate
Language
English
Subjects
Chemistry
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
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