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Learn · Fundamentals of Cryo-Electron Microscopy (Cryo-EM) and 3D Image Processing in Structural Biology

Lecture 56 of 57Week 12 Hands-on Practical Training in TEM & Cryo-EM

Practical: Cryo-EM Grid Clipping

Prof. Somnath Dutta · IISc Bangalore

Credits and licence

From
Fundamentals of Cryo-Electron Microscopy (Cryo-EM) and 3D Image Processing in Structural Biology, IISc Bangalore (at NPTEL)
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
Who can watch
Teens profiles and older. How courses are placed

All the lectures

  1. Introduction of the microscope YouTube
  2. Image formation by glass lenses and its role in wavelength YouTube
  3. Different components of electron microscope (EM) YouTube
  4. Electron specimen interaction YouTube
  5. Elements of TEM: electron gun - I YouTube
  6. Elements of TEM: electron gun - II YouTube
  7. Comparison between the various types of electron gun YouTube
  8. Elements of TEM: electromagnetic lens (EM) - I YouTube
  9. Trajectory of electrons in EM lens YouTube
  10. Elements of TEM: electromagnetic lens (EM) - II YouTube
  11. Image Formation by EM Lenses: Understanding Ray Diagrams YouTube
  12. Aberrations and Focusing in Cryo-EM imaging YouTube
  13. Understanding Chromatic, Spherical Aberrations, and Astigmatism in Cryo-EM YouTube
  14. Aberrations and Practical Imaging in Cryo-EM YouTube
  15. Electron Dose Control and Beam Optimization in Cryo-EM YouTube
  16. Introduction to TEM Holders and Grids in Cryo-EM YouTube
  17. Overfocused and Underfocused Images YouTube
  18. Elements of TEM: Pumps in EM System YouTube
  19. Elements of TEM: Detectors in EM System YouTube
  20. Imaging and Effect of Thermal Drift YouTube
  21. Wave and its properties YouTube
  22. Addition/superposition of waves - I YouTube
  23. Addition/superposition of waves - II YouTube
  24. Decomposition of complex waves YouTube
  25. wave and their transformation YouTube
  26. Complex 2D Images & Its Diffraction Pattern YouTube
  27. Sample Preparation & Determination for TEM YouTube
  28. Biological Sample Purification - I YouTube
  29. Biological Sample Purification - II YouTube
  30. Different Sample Preparation Techniques for TEM Imaging & Different Types of Grids used for Imaging YouTube
  31. TEM Grids: Types, Handling & Surface Coatings YouTube
  32. Carbon Coaters, Glow Discharge & Grid Surface Treatment YouTube
  33. Negative Staining in TEM: Stains, Grid Preparation & Protocols YouTube
  34. Carbon Sandwich & Metal Shadowing Techniques in TEM YouTube
  35. Staining Quality in TEM: Thick, Thin & Positive Staining YouTube
  36. Negative Staining – Imaging Quality & Artifacts YouTube
  37. Negative Staining – Particle Distribution & Contrast YouTube
  38. Applications of Negative Staining in Structural Biology YouTube
  39. Negative Staining for Complexes and Interactions YouTube
  40. Ultramicrotomy and Sectioning for Biological Molecules YouTube
  41. Cryo-EM Grid Preparation & Vitrification YouTube
  42. Grid Types & Sample Distribution in Cryo-EM YouTube
  43. Blotting Parameters & Ice Conditions YouTube
  44. Motion Correction & Defocus in Cryo-EM YouTube
  45. Microscope Alignment & Imaging Limitations YouTube
  46. CTF Estimation & Defocus in Cryo-EM YouTube
  47. Phase Flip & Motion Correction YouTube
  48. High Pressure & Rapid Freezing Methods YouTube
  49. Cryo-Ultra Microtomy & FIB-SEM YouTube
  50. Graphene Oxide Grids in Cryo-EM YouTube
  51. Motion Correction & 2D Classification Basics YouTube
  52. Concepts & Methods in 2D/3D Classification YouTube
  53. 3D Reconstruction & Resolution Assessment YouTube
  54. Practical: TEM Setup & Negative Staining YouTube
  55. Practical: Cryo-EM Plunge Freezing YouTube
  56. Practical: Cryo-EM Grid Clipping YouTube
  57. Practical: Grid Loading & Imaging Software YouTube