Course10210405940 lectures
Cell Culture Technologies
Prof. Mainak Das · IIT Kanpur
About
The course will be a short primer to understand how ‘animal cell culture technologies’ have strengthen the bio-medical research from basic research to the modern drug discovery. Animal cell culture was first performed in the very first decade of 19th century. Since then, tremendous development has taken place in this field. The lectures will help the researcher to appreciate the developments during last hundred years and will help them to independently set up cell culture laboratories. For non-biologist, it will be an informal way to demystify the intriguing routes of biomedical research where cell culture is a very ‘potent tool’.
INTENDED AUDIENCE : UG and PG students pursuing biology, biotechnology, zoology and bio-engineering.
PRE REQUISITE : Biology at standard 10th (Secondary school examination)
INDUSTRY SUPPORT : Biomedical industries, Biotechnology industry, Drug -discovery industry
Lectures
Week 1
- Lecture 01: Introduction of Cell Culture Technology
- Lecture 02: Philosophy and complexity in cell culture
- Lecture 03: To grow the cell outside the body
- Lecture 04: Cell cycle concept
- Lecture 05: Dividing cells
Week 2
- Lecture 06: Biology of cell culture
- Lecture 07: Layout(s) & design(s) of cell culture facility
- Lecture 08: Precautions during designing the lab layout - I
- Lecture 09: Precautions during designing the lab layout - II
- Lecture 10: Precaution during designing the lab out - III
Week 3
- Lecture 11: State of the art facility in cell culture lab - I
- Lecture 12: State of the art facility in cell culture lab - II
- Lecture 13: Specialized facility in cell culture lab
- Lecture 14: Interaction of cell and glass/polycarbonate surface - I
- Lecture 15: Interaction of cell and glass/polycarbonate surface - II
Week 4
- Lecture 16: Poly D lysine deposition
- Lecture 17: Surface chemical analysis
- Lecture 18: Cell growth process
- Lecture 19: Cell surface interface
- Lecture 20: Cell culture substrate patterning
Week 5
- Lecture 21: Introduction to defined system
- Lecture 22: Mechanical dissociation of hippocampal tissue
- Lecture 23: Rules for mechanical dissociation of tissue
- Lecture 24: Drug molecule testing
- Lecture 25: Adult hippocampal neuron dissociation
Week 6
- Lecture 26: Cell separation & In vitro myelination cell culture mode - I
- Lecture 27: Cell separation & In vitro myelination cell culture mode - II
- Lecture 28: Cell separation & In vitro myelination cell culture mode - III
- Lecture 29: Cell Separation & In vitro myelination cell culture mode - IV
- Lecture 30: Cell separation & in vitro myelination cell culture mode - V
Week 7
- Lecture 31: Fluorescent assisted cell sorting
- Lecture 32: Condition for regenerated cells
- Lecture 33: Introduction of skeletal muscle cell culture
- Lecture 34: Skeletal muscle cell culture
- Lecture 35: Cardiac muscle cell culture
Week 8
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IIT Kanpur
- Department
- Biotechnology
- Teacher
- Prof. Mainak Das
- Level
- Graduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
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