14.1: Introduction

Computational Biology - Genomes, Networks, and Evolution · LibreTexts

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    From LibreTexts, under CC BY-NC-SA 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    The purpose of mRNA sequencing (RNA-seq) is to measure the levels of mRNA transcripts for every gene in a given cell. mRNA sequencing was a daunting task, and requires approximately 40 million aligned reads in order to accurately measure mRNA transcripts.This did not become possible until 2009, when next-generation sequencing technologies became more advanced and efficient.

    In this chapter, we will explore the different techniques for using mRNA sequencing data to aid in gene and transcript discovery as well as in expression analysis.

    This page titled 14.1: Introduction was last modified on Sun, 03 Jan 2021 23:16:28 GMT and is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Manolis Kellis et al. (MIT OpenCourseWare) via source content that was edited to the style and standards of the LibreTexts platform.