14.2: Expression Microarrays

Computational Biology - Genomes, Networks, and Evolution · LibreTexts

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    Prior to the development of mRNA sequencing technology, mRNA levels were measured using expression microarrays. These microarrays function by inserting a DNA probe on a slide and measuring the levels transcripts that undergo complimentary hybridization with the DNA, a process that could analyze expression on a gene by gene basis (Figure 1).

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    Figure 14.1: Figure 1: Expression microarray process

    However, this technology has several limitations: it cannot distinguish mRNA isoforms, it cannot analyze on the sequence, or digital level, it can only measure known transcripts, and the expression measurements become less reliable for highly saturated transcript levels.

    This page titled 14.2: Expression Microarrays was last modified on Sun, 03 Jan 2021 23:16:28 GMT and is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Manolis Kellis et al. (MIT OpenCourseWare) via source content that was edited to the style and standards of the LibreTexts platform.