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Strength-of-Neisseria-meningitidis-binding-to-endothelial-cells-requires-highly-ordered-CD147β2-ncomms15764-s4

Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X,…

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Supplementary Movie 3 Actn4 depletion alters CD147 clustering at bacterial adhesion sites.Typical 2D-STORM reconstruction sequence of CD147 distribution at bacterial adhesion sites in Acnt4-depleted cells (related to Fig. 7a). Actn4-depleted HBMECs cells infected for 1 hour with meningococci were fixed and stained for CD147 using an Alexa Fluor 647 conjugated secondary antibody to perform optical super-resolution microscopy technique (dSTORM). 10,000 frames were recorded for each acquisition and reconstructed using QuickPALM 1.1 ImageJ plug with the default settings.

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Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X, Marullo S, Bourdoulous S
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