A-workflow-to-process-3D+time-microscopy-images-of-developing-organisms-and-reconstruct-their-cell-ncomms9674-s11

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Supplementary Movie 10 A single cell is selected at time step 62 (in yellow). Its lineage indicates a single branch with no division. Following its trajectory step by step shows that at time step 71, it actually divides and its color is propagated to only one of its daughters, indicating that the algorithm (SimAnn) did not detect the mitotic event. In the checking mode of the interactive visualization software Mov-IT, the yellow cell at time t70 is linked to its blue daughter at t71. Fixing this link proved being sufficient to recover the complete clonal history of the yellow cell from t62 to the end of the time lapse as it two daughters were otherwise properly tracked.

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Faure E, Savy T, Rizzi B, Melani C, Stašová O, Fabrèges D, Špir R, Hammons M, Čúnderlík R, Recher G, Lombardot B, Duloquin L, Colin I, Kollár J, Desnoulez S, Affaticati P, Maury B, Boyreau A, Nief J, Calvat P, Vernier P, Frain M, Lutfalla G, Kergosien Y, Suret P, Remešíková M, Doursat R, Sarti A, Mikula K, Peyriéras N, Bourgine P

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