Windpipe-Controls-Drosophila-Intestinal-Homeostasis-by-Regulating-JAKSTAT-Pathway-via-Promoting-pgen.1005180.s011

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Wdp expression promotes Dome-GFP trafficking from the cell membrane into the cytoplasm in live S2 cells. (Part 1–3) The dynamics of Dome-GFP in live S2 cells co-transfected with dome-GFP and wdp. Dome-GFP was mainly detected as intracellular punctuates. In Part 1, the arrowheads marked by A, B, C, D and E show the newly formed endocytic vesicles trafficking Dome-GFP from the cell membrane. Part 2 is the enlarged movie of the endocytic vesicles marked by B and C. Part 3 shows the co-localization of Dome-GFP with endocytic dye FM4-64.(Part 4–5) The dynamics of Dome-GFP in live S2 cells transfected with dome-GFP alone. Dome-GFP was mainly present on the cell membrane and no newly formed vesicles trafficking Dome-GFP from the cell membrane were observed (Part 4). In addition, no obvious colocalization of Dome-GFP with FM4-64 was detected in Part 5.

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Ren W, Zhang Y, Li M, Wu L, Wang G, Baeg G, You J, Li Z, Lin X

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