Cell-Cycle–Dependent-Differentiation-Dynamics-Balances-Growth-and-Endocrine-Differentiation-in-the-pbio.1002111.s019

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Time-lapse movie exhibiting asymmetric (P/R) cell division from Pdx1 tTA/+ ; tetO-H2B-GFP;Ngn3-RFP pancreatic explant. After the mother division at elapsed time 09:30:00.000 (white spots), one daughter acquires RFP signal at 19:42:00.000, whereas the other daughter divides at 42:12:00.000. Referring to immunostaining (Fig. 3E,F), the granddaughters are progenitors (SOX9+), demonstrating asymmetric (P/R) division. Left panel, GFP/RFP channels and right panel, RFP channel. Time indicates h:min:s. Frame rate: 6 fps.

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Kim Y, Larsen H, Rué P, Lemaire L, Ferrer J, Grapin-Botton A

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