Capitate type of glandular hairs along the margins of a primrose leaf (Primula sp.)

About

Materials required for preparing the slide:

primrose leaf, microscope slide, cover slip, tweezers, dropper with water, razor blade, filter paper.

Along the margins of primrose leaves, a capitate type of glandular hairs is formed, in which only the terminal cell is glandular and has the ability to produce a secretion. Preparation of this slide begins by placing two drops of water onto a microscope slide: one larger central drop and one smaller peripheral drop. Using a razor blade, a portion of the leaf margin is cut and placed into the central drop of water with the help of tweezers. The cover slip is positioned at a 45° angle starting from the peripheral drop of water and slowly moved until the two drops merge, after which the cover slip is lowered. This method of placing the cover slip is used to prevent air bubbles from being trapped, which would interfere with microscopic observation. If empty air spaces remain under the cover slip, the cover slip is gently pressed with tweezers, and water is carefully added along the edges using a dropper. Excess water is absorbed using filter paper. The microscope slide prepared in this way is ready for observation under the microscope. Under the microscope, glandular hairs along the leaf margins can be observed at different magnifications.

Planned and performed by Sara Cvetanoska, Department of Biology, FNSM, Ss. Cyril and Methodius University, Skopje, Macedonia.

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Wikimedia Commons, in Wikimedia Commons

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CC BY-SA 4.0, as the Wikimedia Commons states it.

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Creative Commons Attribution-Share Alike 4.0

Credit

Deni Ingilizovski

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