Harvesting cultured fat by cell scraping in vitro murine adipocytes

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"Aggregated in vitro adipocytes form macroscale cultured fat tissue constructs After 15–30 days of adipogenesis, cultured adipocytes were harvested with a cell scraper (Figure 3A and B). Adipocytes were scraped mechanically due to insufficient detachment when using Accumax (incubation time: >20 min, data not shown). During cell harvest, cultured adipocytes aggregated by the mechanical action of the cell scraper appeared like fat tissue or lipoaspirate (Figure 3—figure supplement 1, Video 2)."

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Creative Commons Attribution 4.0

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Authors of the study: John Se Kit Yuen Jr, Michael K Saad, Ning Xiang, Brigid M Barrick, Hailey DiCindio, Chunmei Li, Sabrina W Zhang, Miriam Rittenberg, Emily T Lew, Kevin Lin Zhang, Glenn Leung, Jaymie A Pietropinto, David L Kaplan

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