Quantitative NMR

by Cynthia K. Larive & Albert K. Korir

Read here Read at LibreTextsPDF

Quantitative nuclear magnetic resonance (Q-NMR) measurements are especially useful for analysis of complex samples where high specificity is needed or when a pure standard of the analyte is not available. This text provides a brief overview of basic NMR theory, a tutorial discussing the practical aspects of Q-NMR, a virtual experiment utilizing an NMR simulator, an exercise that makes use of real NMR data you can download and analyze, and a Q-NMR lab experiments.

Description from LibreTexts.

Publisher
University of California, Riverside & Drury University
Subjects
Chemistry, Physical and Theoretical Chemistry Textbook Maps
Licence
CC BY-NC-SA 2.5, as LibreTexts states it. Non-commercial use only.
Who can read
Tweens profiles and older, judged from its title and subjects

Contents

LibreTexts writes equations in TeX, and here they show as TeX; the PDF has them typeset.

  1. Front Matter
  2. TitlePage
  3. InfoPage
  4. About this Book
  5. Table of Contents
  6. Licensing
  7. 1: Basic NMR Theory
  8. 1.1: What is spin?
  9. 1.2: How does absorption of energy generate an NMR spectrum?
  10. 1.3: How does the population difference in NMR compare to the difference between electronic ground and excited states?
  11. 1.4: What is chemical shift and how does it relate to resonance frequency?
  12. 1.5: What is Precession?
  13. 1.6: How does precession generate the macroscopic magnetization (Mo)?
  14. 1.7: How can the nuclear spins be manipulated to generate the NMR spectrum?
  15. 1.8: What is the tip angle?
  16. 1.9: What is the Free Induction Decay?
  17. 1.10: How do T₁ and T₂ relaxation affect NMR spectra?
  18. 1.11: Where should I look to learn more about NMR?
  19. 2: Practical Aspects of Q-NMR
  20. 2.1: How do I choose a reference standard for my Q-NMR analysis?
  21. 2.2: How is the internal standard used to quantify the concentration of my analyte?
  22. 2.3: What sample considerations are important?
  23. 2.4: How do I choose the right acquisition parameters for a quantitative NMR measurement?
  24. 2.5: Effects of Tip Angle in Quantitative NMR Experiments
  25. 2.6: What data processing considerations are important for obtaining accurate and precise results?
  26. 2.7: References
  27. 3: Virtual Experiment
  28. 3.1: Virtual Laboratory
  29. 4: Q-NMR Experiment
  30. 4.1: Prelab Exercises
  31. 4.2: Background
  32. 4.3: Dry Lab
  33. 4.4: Wet Lab
  34. 5: Q-NMR Applications
  35. 5.1: Q-NMR for purity determination of macrolide antibiotic reference standards- Comparison with the mass balance method
  36. 5.2: Determining Enantiomeric or Isomeric Purity of Active Pharmaceutical Ingredients
  37. 5.3: Q-NMR for Analysis and Characterization in Vaccine Preparations
  38. 5.4: Q-NMR-Based Metabonomics of Blood Samples
  39. 5.5: Q-NMR for Time Course Evolution of Malic and Lactic Acid
  40. 6: Instructor's Guide
  41. 6.1: Basic Theory Concept Questions
  42. 6.2: Answers to Questions in the Basic Theory section
  43. 6.3: Practical Aspects Concept Questions
  44. 6.4: Answers to Questions in the Practical Aspects section
  45. 6.5: Q-NMR Drylab
  46. Back Matter
  47. Index
  48. Advanced Theory
  49. Detailed Licensing