Yours

2.1.8.7: Cheek Cell DNA Extraction

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    From LibreTexts, under CC BY-NC-SA 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Using Cytobrush

    1. Use a sterile cytobrush and insert into mouth.
    2. Brush the cytobrush on the inside of your cheek 25 times.
    3. Swirl the cytobrush in 100 μl of Chelex suspension (10% w/v).
    4. Place a centrifuge tube with Chelex and cell suspension on a 100 °C heat block for 10 minutes.
    5. Centrifuge the tubes at maximum speed for 5 minutes.
    6. DNA is in the supernatant. Avoid beads at the bottom.
    7. Store DNA at -20 °C.

    PCR with PCR Beads

    1. Add 22 μl of the primer mix (forward and reverse) to beads.
    2. Ensure that the bead is dissolved.
    3. Add 3 μl of DNA.

    This page titled 2.1.8.7: Cheek Cell DNA Extraction was last modified on Mon, 28 Aug 2023 17:39:32 GMT and is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Shannon Clark.