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8.3: Pull Sheets/Materials

Bio 4A+4B Lab Instructor Resources Manual · LibreTexts

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    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

     Item How many?
    Lab coats (borrow boxes from physiology)         28
    Goggles (large ones for people with glasses) 28
    Bacteria “3 types” microscope slides (cocci, bacilli, spirilla) 28
    Poster - Bacteria Morphological Types 1
    Bacterium model 1
    Kimwipes 2 boxes
    Bacterial clearning solutions (Wavicide) 6 bottles
    Paper towel rolls 6
    Plain microscope slides 2 boxes
    Clean or sterile toothpicks 1 container
    Dropper bottle of DI water 6
    DI wash bottles 6
    Lens paper packets 6
    Immersion oil 6 dropper bottles
    Test tube racks (yellow ones) 12
    Inoculating loops 12
    Bunsen burners 6
    Striker lighters 6
    Wax pencils 6
    Staining trays and racks 12
    Bibulous paper 12
    Clothespin 12

    Solutions for Gram staining in boxes

    - Gram's crystal violet

    -Gram's iodine solution

    -95% ethanol

    -Safranin stain

    6-12 staining boxes
    Beaker with 10% bleach for used toothpicks 1
    Large autoclave waste bag for used gloves 1 per lab section
    Soak container for used slides 1
    Waste container for used Gram stain (in hood) 1
    Gloves (s, m, l) for handling bacteria  1 box of each
    *Quadrant streak plate of Micrococcus luteus                             3 (1 per lab table) parafilm to keep closed
    *Quadrant streak plate of Serratia marcescens (Grown at 25C for red color) 3 (1 per lab table) parafilm to keep closed
    *Quadrant streak plate of Bacillus 3 (1 per lab table) parafilm to keep closed
    *Quadrant streak plate of E. coli 3 (1 per lab table) parafilm to keep closed
    *Slant culture of Micrococcus luteus 6 (3 per lab section)
    *Slant culture of Serratia marcescens (Grown at 25 for red color) 6 (3 per lab section)
    *Slant culture of Bacillus 6 (3 per lab section)
    *Slant culture of E. coli 6 (3 per lab section)
    Sterile TSA agar plates  14 per lab section
    Sterile cotton swabs 28 per lab section
    3 mL sterile water in 16mm test tube 14 per lab section
    • All of these cultures should be available from our microbiology tech (also used for Biol74)

     

    This page titled 8.3: Pull Sheets/Materials was last modified on Tue, 27 Aug 2024 07:44:28 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Darcy Ernst, May Chen, Katie Foltz, and Bridget Greuel (Open Educational Resource Initiative at Evergreen Valley College) .