13.3: Pull Sheets/Materials

Bio 4A+4B Lab Instructor Resources Manual · LibreTexts

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    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

     Item How many?
    Gloves (s, m, l) 1 box of each

    Spinach leaves:

    • Dark, green, fresh leaves are best
    • Each lab section needs approx. 25g leaves

    2 bundles (1 / lab section)

    or at least 80 leaves (40 / lab section)
    Electronic balance 6 - 2 per lab bench
    Weighing boats for balance 1 at each balance

    Clean quartz sand (to grind spinach)

    • 0.4g per "pinch"
    • 24 pinches (12 pinches/lab section)
    		 10 grams (5 g / lab section)
    Mortar and pestle 6 per lab section
    Acetone (keep in hood) 80 mL (40 mL / lab section)
    Test tubes 24 (12 / lab section)
    Test tube racks 6
    Chromatography papers (11 cm square or similar) 12 (6 / lab section)
    Glass capillary tubes 12 (6 / lab section)
    Grass or spinach soaking in acetone (for student demo) 1
    Extra 6" rulers 6
    Pencils 6
    Handheld UV light  1 (2 preferred)

    With each stored in 1 L glass jar with lid:

    • *petroleum ether-acetone solution

    6 (placed in safety hood)
    Special waste container for acetone/chlorophyll extract 1 (placed in safety hood)

    3 different potted plants with flat leaves

    • Best if 3 plants have leaves of different colors

    		 each plant should have 14 leaves
    95% ethanol 250 mL (125 mL / lab section)
    Spectrophotometers 6 – 2 per lab bench
    Plastic cuvettes 24 (12 / lab section)
    Pasteur pipettes 36 (18 / lab section)
    10 or 20 mL plastic graduated cylinders 6 (rinse/replace between lab sections)
    Clear plastic cups (to hold at least 300mL) 24 (12/lab section)
    Index cards 24 (12/lab section)
    Plastic petri dishes 12 (rinse/replace between lab sections)
    Water with 0.3%-0.5 sodium bicarbonate (NaHCO3) or baking soda 1000 mL (500 mL/lab section)
    100 mL beaker 6 (rinse/replace between lab sections)    

    Dilute detergent (5mL dishwashing detergent+250mL water)

    • Aliquote into microcentrifuge tubes, label “DD”

    at least 12 microcentrifuge tubes 

    (6 per lab section)
    Hole puncher (or straws) 6 (or 12 straws for both lab sections)
    10 mL syringes without needles 12 (rinse/replace between lab sections)
    Stopwatches 6
    Desk lamps 6 preferred (3 is okay)
    Bottle of clear nail polish 6 preferred (3 is okay)
    Clear packing tape 6 rolls
    Microscope slides 1 box

     

    *Preparation for the Petroleum ether-acetone solution: Use 9 parts petroleum ether and 1 part acetone; volume should be less than 1” in height from bottom of each jar.

    This page titled 13.3: Pull Sheets/Materials was last modified on Tue, 27 Aug 2024 07:44:34 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Darcy Ernst, May Chen, Katie Foltz, and Bridget Greuel (Open Educational Resource Initiative at Evergreen Valley College) .