# Strength-of-Neisseria-meningitidis-binding-to-endothelial-cells-requires-highly-ordered-CD147β2-ncomms15764-s4

Supplementary Movie 3 Actn4 depletion alters CD147 clustering at bacterial adhesion sites.Typical 2D-STORM reconstruction sequence of CD147 distribution at bacterial adhesion sites in Acnt4-depleted cells (related to Fig. 7a). Actn4-depleted HBMECs cells infected for 1 hour with meningococci were fixed and stained for CD147 using an Alexa Fluor 647 conjugated secondary antibody to perform optical super-resolution microscopy technique (dSTORM). 10,000 frames were recorded for each acquisition and reconstructed using QuickPALM 1.1 ImageJ plug with the default settings.

- Kind: Short
- Year: 2017
- Runtime: 2 min
- By: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X,…
- Archive: [Wikimedia Commons](https://commons.wikimedia.org/wiki/File:Strength-of-Neisseria-meningitidis-binding-to-endothelial-cells-requires-highly-ordered-CD147%CE%B22-ncomms15764-s4.ogv)
- Licence or status: [CC BY 4.0](https://creativecommons.org/licenses/by/4.0) (as the archive states it)
- Credit: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X, Marullo S, Bourdoulous S
- Who can watch: Teens profiles and older (the archive gives no rating; placed by Invisible Network from its collection and subjects)

[Watch now](https://invisiblenetwork.ai/watch/archive/commons/60246202?play=1)