# Fluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s010

Video animation of RABV ΔG-EGFP (EnvA) infected neurons in EC showing axon traces of monosynaptically connected neurons. Right hemisphere horizontal dataset (same as shown in Fig 4) was downsampled to 6.45 μm cubic voxel size and converted from 16 bit greyscale TIFF into 8 bit Amira Mesh format. Four 3D traces of axons manually generated with KNOSSOS software were overlaid as color-coded, solid lines with the EGFP channel (color-coded grey) for generating part of the animation. The trajectory of one of the axons traced with KNOSSOS is depicted by a series of red dots after zooming in to the original 1.61 x 1.61 μm xy pixel size. Note the autofluorescence of the brain periphery.

- Kind: Short
- Year: 2015
- Runtime: 2 min
- By: Schwarz M, Scherbarth A, Sprengel R, Engelhardt J, Theer P, Giese G
- Archive: [Wikimedia Commons](https://commons.wikimedia.org/wiki/File:Fluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s010.ogv)
- Licence or status: [CC BY 4.0](https://creativecommons.org/licenses/by/4.0) (as the archive states it)
- Credit: Schwarz M, Scherbarth A, Sprengel R, Engelhardt J, Theer P, Giese G
- Who can watch: Teens profiles and older (the archive gives no rating; placed by Invisible Network from its collection and subjects)

[Watch now](https://invisiblenetwork.ai/watch/archive/commons/40637879?play=1)