# In-Silico-Single-Molecule-Manipulation-of-DNA-with-Rigid-Body-Dynamics-pcbi.1003456.s007

Illustration of the sampling efficiency of global versus local thermostat. This video records the simulated dynamics of a DNA molecule manipulated by magnetic tweezers after thermal equilibrium has been reached. The stretching force is pN and the number of turns of the bead is fixed to . From to s the simulation is run with Langevin local thermostat. Then the global thermostat is started up at s and run until s, strikingly accelerating the dynamics of the DNA molecule. Moreover the plectoneme starts to diffuse along the DNA molecule. Then the local thermostat is run again from s to , resulting in a dramatic slowing down of the dynamics. And finally the global thermostat is started up again at and run until the end of the video at . The DNA molecule is composed of rigid cylinders of radius and . The other parameters of the simulation are given in Table 1. This video is also available at http://vimeo.com/51918121.

- Kind: Short
- Year: 2014
- Runtime: 1 min
- By: Carrivain P, Barbi M, Victor J
- Archive: [Wikimedia Commons](https://commons.wikimedia.org/wiki/File:In-Silico-Single-Molecule-Manipulation-of-DNA-with-Rigid-Body-Dynamics-pcbi.1003456.s007.ogv)
- Licence or status: [CC BY 4.0](https://creativecommons.org/licenses/by/4.0) (as the archive states it)
- Credit: Carrivain P, Barbi M, Victor J
- Who can watch: Teens profiles and older (the archive gives no rating; placed by Invisible Network from its collection and subjects)

[Watch now](https://invisiblenetwork.ai/watch/archive/commons/31384008?play=1)