# Harvesting cultured fat by cell scraping in vitro murine adipocytes

"Aggregated in vitro adipocytes form macroscale cultured fat tissue constructs After 15–30 days of adipogenesis, cultured adipocytes were harvested with a cell scraper (Figure 3A and B). Adipocytes were scraped mechanically due to insufficient detachment when using Accumax (incubation time: >20 min, data not shown). During cell harvest, cultured adipocytes aggregated by the mechanical action of the cell scraper appeared like fat tissue or lipoaspirate (Figure 3—figure supplement 1, Video 2)."

- Kind: Short
- Year: 2023
- Runtime: 1 min
- By: Authors of the study: John Se Kit Yuen Jr, Michael K Saad, Ning Xiang, Brigid M Barrick, Hailey DiCindio, Chunmei Li, Sabrina W Zhang, Miriam Rittenberg,…
- Genres: Science
- Archive: [Wikimedia Commons](https://commons.wikimedia.org/wiki/File:Harvesting_cultured_fat_by_cell_scraping_in_vitro_murine_adipocytes.webm)
- Licence or status: [CC BY 4.0](https://creativecommons.org/licenses/by/4.0) (as the archive states it)
- Credit: Authors of the study: John Se Kit Yuen Jr, Michael K Saad, Ning Xiang, Brigid M Barrick, Hailey DiCindio, Chunmei Li, Sabrina W Zhang, Miriam Rittenberg, Emily T Lew, Kevin Lin Zhang, Glenn Leung, Jaymie A Pietropinto, David L Kaplan
- Who can watch: Tweens profiles and older (the archive gives no rating; placed by Invisible Network from its collection and subjects)

[Watch now](https://invisiblenetwork.ai/watch/archive/commons/132803055?play=1)