# Experimental Biochemistry

- Institution: IIT Kharagpur
- Department: Chemistry and Biochemistry
- Taught by: Prof. Swagata Dasgupta, Prof. Soumya De
- Level: Undergraduate
- Language: English
- Subjects: Chemistry
- Source: [NPTEL](https://nptel.ac.in/courses/104105102)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/104105102)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

## Lectures

1. [Lecture 1 : Introduction](https://invisiblenetwork.ai/learn/nptel/104105102/81b7ca130755) (Video)
2. [Lecture 2 : Buffers](https://invisiblenetwork.ai/learn/nptel/104105102/b73da8483e38) (Video)
3. [Lecture 3 Introduction to Biochemistry Laboratory Equipments and Safety Measures](https://invisiblenetwork.ai/learn/nptel/104105102/c70ee483e01e) (Video)
4. [Lecture 4 Practical Aspects of Making Buffer](https://invisiblenetwork.ai/learn/nptel/104105102/638a75743282) (Video)
5. [Lecture 5 Making Tris Buffer (pH=8.2)](https://invisiblenetwork.ai/learn/nptel/104105102/236b01b8da6d) (Video)
6. [Lecture 6 Making Phosphate Buffer (100mM)](https://invisiblenetwork.ai/learn/nptel/104105102/5d6b5d039ca9) (Video)
7. [Lecture 07 : Amino Acids and Their Properties](https://invisiblenetwork.ai/learn/nptel/104105102/bba43456d5e0) (Video)
8. [Lecture 08 : Amino Acid Titrations](https://invisiblenetwork.ai/learn/nptel/104105102/d501eb5cbc0b) (Video)
9. [Lecture 09 : pI Determination of Glycine](https://invisiblenetwork.ai/learn/nptel/104105102/04b5a9efb84d) (Video)
10. [Lecture 10 : pI Determination of Lysine](https://invisiblenetwork.ai/learn/nptel/104105102/66cc100d3858) (Video)
11. [Lecture 11 : Summary](https://invisiblenetwork.ai/learn/nptel/104105102/15d28b2fcac7) (Video)
12. [Lecture 12 : UV and Visible Spectroscopy](https://invisiblenetwork.ai/learn/nptel/104105102/55b8f1ed124c) (Video)
13. [Lecture 13 : Fluorescence Spectroscopy](https://invisiblenetwork.ai/learn/nptel/104105102/d39e542f0f94) (Video)
14. [Lecture 14 : UV/Visible Spectra of Amino Acids and Proteins](https://invisiblenetwork.ai/learn/nptel/104105102/f542c27f3ae7) (Video)
15. [Lecture 15 : Fluorescence Spectra of Amino Acids and proteins](https://invisiblenetwork.ai/learn/nptel/104105102/09d1d57ae715) (Video)
16. [Lecture 16 : Spectroscopic Techniques Summary](https://invisiblenetwork.ai/learn/nptel/104105102/18ec36a1c7d6) (Video)
17. [Lecture 17 : Protein Folding and Denaturation - I](https://invisiblenetwork.ai/learn/nptel/104105102/9f7a61792da1) (Video)
18. [Lecture 18 : Protein Folding and Denaturation - II](https://invisiblenetwork.ai/learn/nptel/104105102/fdac66f9c24d) (Video)
19. [Lecture 19 : Urea denaturation of HSA studied by UV/Vis absorbance](https://invisiblenetwork.ai/learn/nptel/104105102/dc8878590edd) (Video)
20. [Lecture 20 : Temperature denaturation of HSA studied by UV/Vis absorbance](https://invisiblenetwork.ai/learn/nptel/104105102/84f24468d23e) (Video)
21. [Lecture 21 : Denaturation of HSA by GdnHCl studied by Trp fluorescence](https://invisiblenetwork.ai/learn/nptel/104105102/018f0ab2db74) (Video)
22. [Lecture 22 : Protein Folding and Denaturation Summary](https://invisiblenetwork.ai/learn/nptel/104105102/dd9f3659c9ca) (Video)
23. [Lecture 23 : Chromatographic Techniques - I](https://invisiblenetwork.ai/learn/nptel/104105102/123f63aceaf7) (Video)
24. [Lecture 24 : Chromatographic Techniques - II](https://invisiblenetwork.ai/learn/nptel/104105102/a42316d7b92e) (Video)
25. [Lecture 25 : Protein Purification by Size Exclusion Chromatography (SEC)](https://invisiblenetwork.ai/learn/nptel/104105102/a8f81c9d7e83) (Video)
26. [Lecture 26 : Protein Purification by Affinity Chromatography](https://invisiblenetwork.ai/learn/nptel/104105102/a37584ccbd95) (Video)
27. [Lecture 27: Gel Electrophoresis of DNA and Proteins - Part I](https://invisiblenetwork.ai/learn/nptel/104105102/7c76d7bddfff) (Video)
28. [Lecture 28: Gel Electrophoresis of DNA and Proteins - Part II](https://invisiblenetwork.ai/learn/nptel/104105102/63e1edfa2eb9) (Video)
29. [Lecture 29: Gel Electrophoresis of DNA and Proteins - Part II](https://invisiblenetwork.ai/learn/nptel/104105102/5ce18326a360) (Video)
30. [Lecture 30 : Isolation and Characterization of Proteins Part - I](https://invisiblenetwork.ai/learn/nptel/104105102/2584beb1dc44) (Video)
31. [Lecture 31 : Isolation and Characterization of Proteins Part - II](https://invisiblenetwork.ai/learn/nptel/104105102/ee6c56ad270d) (Video)
32. [Lecture 32 : Isolation and Purification of Proteins](https://invisiblenetwork.ai/learn/nptel/104105102/07f677159ba7) (Video)
33. [Lecture 33 : Quality and Quantity of the Isolated Protein](https://invisiblenetwork.ai/learn/nptel/104105102/4d0ef7734167) (Video)
34. [Lecture 34 : Enzyme Kinetics I](https://invisiblenetwork.ai/learn/nptel/104105102/ef8d6529e575) (Video)
35. [Lecture 35 : Enzyme Kinetics II](https://invisiblenetwork.ai/learn/nptel/104105102/1311fa04394b) (Video)
36. [Lecture 36 : Enzyme Kinetics (by using enzyme from apple juice)](https://invisiblenetwork.ai/learn/nptel/104105102/14a9d1b7fb60) (Video)
37. [Lecture 37 : Enzyme Kinetics (by using enzyme from apple juice) (Contd.)](https://invisiblenetwork.ai/learn/nptel/104105102/aba71df03923) (Video)
38. [Lecture 38 : Isolation and Characterization of DNA Part - I](https://invisiblenetwork.ai/learn/nptel/104105102/2b67a0bae5d8) (Video)
39. [Lecture 39 : Isolation and Characterization of DNA Part - II](https://invisiblenetwork.ai/learn/nptel/104105102/02283be79fcd) (Video)
40. [Lecture 40 : Bacterial Culture for Plasmid DNA Isolation](https://invisiblenetwork.ai/learn/nptel/104105102/09509727fb73) (Video)
41. [Lecture 41 : Isolation of Plasmid DNA](https://invisiblenetwork.ai/learn/nptel/104105102/d2347b79d02b) (Video)
42. [Lecture 42 : Isolation and Characterization of DNA Summary](https://invisiblenetwork.ai/learn/nptel/104105102/9d923e8e2b7b) (Video)
43. [Lecture 43 : Basics of rDNA Technology Part - I](https://invisiblenetwork.ai/learn/nptel/104105102/fd296b640bed) (Video)
44. [Lecture 44 : Basics of rDNA Technology Part - II](https://invisiblenetwork.ai/learn/nptel/104105102/95d23c026536) (Video)
45. [Lecture 45 : Cloning : Polymerase Chain Reaction, Restriction Enzyme Digestion and Ligation](https://invisiblenetwork.ai/learn/nptel/104105102/63adc996f1fc) (Video)
46. [Lecture 46 : DNA Transformation](https://invisiblenetwork.ai/learn/nptel/104105102/cc8530d236a9) (Video)
47. [Lecture 47 : Protein-Ligand Interaction](https://invisiblenetwork.ai/learn/nptel/104105102/b44db9ec2f05) (Video)
48. [Lecture 48 : Protein-Ligand Interaction (Contd.)](https://invisiblenetwork.ai/learn/nptel/104105102/6e081dde92db) (Video)
49. [Lecture 49 : Interaction study of HSA protein with Curcumin and Gallic acid using UV-Vis spectroscopy](https://invisiblenetwork.ai/learn/nptel/104105102/4018de12027b) (Video)
50. [Lecture 50 : Interaction study of HSA protein with Curcumin and Gallic acid using UV-Vis spectroscopy (Contd.)](https://invisiblenetwork.ai/learn/nptel/104105102/8eba4f8df14f) (Video)
51. [Lecture 51 : Analysis of the structure of protein ligand complex](https://invisiblenetwork.ai/learn/nptel/104105102/cd31057a1d35) (Video)
52. [Lecture 52: Immunoassay Techniques](https://invisiblenetwork.ai/learn/nptel/104105102/2415b0409961) (Video)
53. [Lecture 53: Western Blotting Technique](https://invisiblenetwork.ai/learn/nptel/104105102/7e46ac5982c8) (Video)