# Animal Transgenesis: Principles and Methods

- Institution: IISER Bhopal
- Department: Biotechnology
- Teacher: Prof. Himanshu Kumar
- Level: Undergraduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102106821)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102106821)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

## Lectures

1. [Common terms used in the course](https://invisiblenetwork.ai/learn/nptel/102106821/1c66a1418f20) (Video)
2. [Why transgenesis is needed?](https://invisiblenetwork.ai/learn/nptel/102106821/fc858261913f) (Video)
3. [Model organisms for transgenesis](https://invisiblenetwork.ai/learn/nptel/102106821/07ee95124671) (Video)
4. [3R Repalace Reduce Refine](https://invisiblenetwork.ai/learn/nptel/102106821/b00627d7e07f) (Video)
5. [History of transgensis-I](https://invisiblenetwork.ai/learn/nptel/102106821/dc60a01ce0e1) (Video)
6. [History of transgensis-II](https://invisiblenetwork.ai/learn/nptel/102106821/bb53505342ec) (Video)
7. [General Principles of Transgenesis in Mouse](https://invisiblenetwork.ai/learn/nptel/102106821/b3408166aa2e) (Video)
8. [History of Cell culture](https://invisiblenetwork.ai/learn/nptel/102106821/e1b8b85ce3ba) (Video)
9. [Cell culture Types & Basic Requirements](https://invisiblenetwork.ai/learn/nptel/102106821/599285016e66) (Video)
10. [Culture medium and Challenges with cell culture](https://invisiblenetwork.ai/learn/nptel/102106821/474eb1f8f4b8) (Video)
11. [Methods for internalization of transgene, & Cell Preservation & Generation of transgene overexpressing mouse](https://invisiblenetwork.ai/learn/nptel/102106821/92fb602a2cf3) (Video)
12. [Cell for transgenesis and Homologus recombination](https://invisiblenetwork.ai/learn/nptel/102106821/4666a9fd5e4b) (Video)
13. [Targeting vector](https://invisiblenetwork.ai/learn/nptel/102106821/edddebd5fa47) (Video)
14. [ES cells, Electroporation, Screening, & Founder or Chimera mice](https://invisiblenetwork.ai/learn/nptel/102106821/e25191de0377) (Video)
15. [Germline transmission & Conditional knockout mice](https://invisiblenetwork.ai/learn/nptel/102106821/29025c060c55) (Video)
16. [Example of conditional knockout mice & Temporal control of gene expression](https://invisiblenetwork.ai/learn/nptel/102106821/b393201379f8) (Video)
17. [Discovery, origin and Types of CRISPR-Cas9 System](https://invisiblenetwork.ai/learn/nptel/102106821/10be93f5a9a5) (Video)
18. ["CRISPR-Cas9 sgRNA and it’s modifications for gene editing"](https://invisiblenetwork.ai/learn/nptel/102106821/cece11c1252e) (Video)
19. [CRISPR-Cas9 version and it’s application](https://invisiblenetwork.ai/learn/nptel/102106821/657eaf94da36) (Video)
20. ["Genome-wide CRISPR-Cas9 screening and its variants application"](https://invisiblenetwork.ai/learn/nptel/102106821/963229d87f9f) (Video)
21. [CRISPR-Cas9 in therapeutics](https://invisiblenetwork.ai/learn/nptel/102106821/2bbd046af5c8) (Video)