# Mass Spectrometry and its application in Molecular Medicine - Institution: IISER Kolkata - Department: Biotechnology - Teacher: Prof. Amit Kumar Mandal - Level: Undergraduate - Language: English - Subjects: Biology - Source: [NPTEL](https://nptel.ac.in/courses/102106818) - Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.) - Syllabus: [at the source](https://nptel.ac.in/courses/102106818) - Who can watch: Teens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words) ## Lectures 1. [Introduction to soft ionisation mass spectrometry: ESI-MS and MALDI-MS](https://invisiblenetwork.ai/learn/nptel/102106818/1bdf37f20fee) (Video) 2. [Isotopic distribution of signals in mass spectra and deconvolution of mass spectra](https://invisiblenetwork.ai/learn/nptel/102106818/a7e92bac18ae) (Video) 3. [Resolution of mass spectra, accuracy in mass measurement and introduction of molecular medicine](https://invisiblenetwork.ai/learn/nptel/102106818/5ee2a29b6454) (Video) 4. [Mass spectra of human hemoglobin and glycated hemoglobin, a diagnostic marker of diabetes mellitus and glutathionyl hemoglobin, a marker of oxidative stress](https://invisiblenetwork.ai/learn/nptel/102106818/41c6fb22a742) (Video) 5. [Mass spectrometry based quantification of oxidative stress in patients with chronic renal failure](https://invisiblenetwork.ai/learn/nptel/102106818/9881687d7c19) (Video) 6. [Introduction to tandem mass spectrometry and principle of de novo sequencing](https://invisiblenetwork.ai/learn/nptel/102106818/9dc770a9f9ff) (Video) 7. [Chemical modification and enzyme digestion of a peptide: A step towards de novo sequencing](https://invisiblenetwork.ai/learn/nptel/102106818/58dc7c3a1d1d) (Video) 8. [De novo sequencing of a peptide from chemical derivatization and tandem mass spectra](https://invisiblenetwork.ai/learn/nptel/102106818/609bc65542df) (Video) 9. [De novo sequencing: Identification of isobaric amino acid and disulfide connectivity](https://invisiblenetwork.ai/learn/nptel/102106818/b8248a004a37) (Video) 10. [De novo sequencing of a tryptic peptide from tandem mass spectra](https://invisiblenetwork.ai/learn/nptel/102106818/bf38c33ad3a8) (Video) 11. [De novo sequencing from tandem-MS and SPITC derivatization to simplify tandem-MS](https://invisiblenetwork.ai/learn/nptel/102106818/c88f23534f69) (Video) 12. [Routine diagnosis of Hemoglobinopathy: A disease caused by genetic variant of hemoglobin](https://invisiblenetwork.ai/learn/nptel/102106818/74880a6ded04) (Video) 13. [Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-1](https://invisiblenetwork.ai/learn/nptel/102106818/ae078a31e9f1) (Video) 14. [Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-2](https://invisiblenetwork.ai/learn/nptel/102106818/7ece83558654) (Video) 15. [A genetic variant of hemoglobin causing false elevation of HbA1c and hence false diagnosis of diabetes mellitus](https://invisiblenetwork.ai/learn/nptel/102106818/3b2f82e11d0c) (Video) 16. [Nano-LC-ESI-MS based analysis of a proteome - A bottom up proteomics approach](https://invisiblenetwork.ai/learn/nptel/102106818/4224ba2821dc) (Video) 17. [Iron salts as food fortificants to combat Iron Deficiency Anemia](https://invisiblenetwork.ai/learn/nptel/102106818/632e06320a23) (Video) 18. [Nano ferric pyrophosphate as a food fortificant in a rat model: Bioavailability and toxicity assessment](https://invisiblenetwork.ai/learn/nptel/102106818/a3ac569533fa) (Video) 19. [Nano ferric pyrophosphate as a food fortificant in a rat model: Toxicity assessment using biochemical, histopathological and proteomics approach](https://invisiblenetwork.ai/learn/nptel/102106818/53dfa7d12175) (Video) 20. [Nano ferric pyrophosphate as a food fortificant in a rat model: Exploring biomarker of nano particle induced toxicity in rat plasma](https://invisiblenetwork.ai/learn/nptel/102106818/e50e1576dbba) (Video) 21. [Biomarker of nano particles induced toxicity in a rat model: NanoLC-ESI-MS based plasma proteomics approach](https://invisiblenetwork.ai/learn/nptel/102106818/a877ef04e0c3) (Video) 22. [Quantitative proteomics of rat plasma proteins: Absolute quantification of Fetuin B, a biomarker of nano particle toxicity](https://invisiblenetwork.ai/learn/nptel/102106818/92ea8b345322) (Video) 23. [2D gel electrophoresis based proteomics: principles and methods](https://invisiblenetwork.ai/learn/nptel/102106818/fb9cc9c7ea47) (Video) 24. [Differential plasma proteomics: 2D gel electrophoresis based approach](https://invisiblenetwork.ai/learn/nptel/102106818/ffd7234879f5) (Video) 25. [Analysis of 2D gel spots followed by in-gel digestion to identify proteins usng MALDI-MS platform](https://invisiblenetwork.ai/learn/nptel/102106818/cca52b088490) (Video) 26. [Mass spectrometry based absolute quantification of human plasma proteins](https://invisiblenetwork.ai/learn/nptel/102106818/0c1ff65044b3) (Video) 27. [Absolute quantification of plasma Apolipoprotein-AIV: A probale biomarker of attempted suicide](https://invisiblenetwork.ai/learn/nptel/102106818/d4298053962b) (Video) 28. [MALDI-MS based identification of microorganism in clinical samples](https://invisiblenetwork.ai/learn/nptel/102106818/2600a34684ea) (Video) 29. [Native mass spectrometry using nano-ESI-MS platform](https://invisiblenetwork.ai/learn/nptel/102106818/d9d18daf8e18) (Video) 30. [Analysis of an array of glycated hemoglobin in patients with poorly controlled diabetes mellitus](https://invisiblenetwork.ai/learn/nptel/102106818/79078443e85d) (Video) 31. [Molecular mechanism of sickle hemoglobin polymerisation](https://invisiblenetwork.ai/learn/nptel/102106818/9ccb137e1fb9) (Video) 32. [Inhibition of sickle hemoglobin polymerisation upon glutathionylation](https://invisiblenetwork.ai/learn/nptel/102106818/b17cbb44c872) (Video) 33. [Structural analysis of desorbed hemoglobin from nano particle surface](https://invisiblenetwork.ai/learn/nptel/102106818/774550f20e04) (Video) 34. [Functional assay of human hemoglobin and its variants](https://invisiblenetwork.ai/learn/nptel/102106818/fcbcb83e73e3) (Video) 35. [Application of native mas spectrometry to calculate equilibrium constant](https://invisiblenetwork.ai/learn/nptel/102106818/3de23b58328a) (Video) 36. [Bioconjugation of para-benzoquinone of cigarette smoke with human hemoglobin](https://invisiblenetwork.ai/learn/nptel/102106818/bd55821aeac6) (Video) 37. [Bioconjugation of human hemoglobin with quinone as airborne enviroment pollutants](https://invisiblenetwork.ai/learn/nptel/102106818/703379facb88) (Video) 38. [Ion mobility mass spectrometry](https://invisiblenetwork.ai/learn/nptel/102106818/a16a283e6d03) (Video) 39. [Introduction to hydrogen-deuterium-exchange mass spectrometry](https://invisiblenetwork.ai/learn/nptel/102106818/101905bf58f5) (Video) 40. [Rate law of hydrogen-deuterium-exchange kinetics](https://invisiblenetwork.ai/learn/nptel/102106818/0b936297c525) (Video) 41. [Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-I](https://invisiblenetwork.ai/learn/nptel/102106818/ff0d23d5cd12) (Video) 42. [Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-II](https://invisiblenetwork.ai/learn/nptel/102106818/5e723adc0ae1) (Video) 43. [Molecular insights in inhibition of HbS polymerisation upon oxygenation -part-III- and upon glutathionylation-part-I-monitored by HDX-MS platform](https://invisiblenetwork.ai/learn/nptel/102106818/8fc6bbf9af46) (Video) 44. [Molecular insights in inhibition of HbS polymerisation upon glutathionylation-part-II](https://invisiblenetwork.ai/learn/nptel/102106818/3b3795b9f385) (Video) 45. [N-acetyl cysteine as an antisickling agent in sickle cell disease](https://invisiblenetwork.ai/learn/nptel/102106818/aff19334b343) (Video) 46. [Effect of quinones on morphology of human RBC](https://invisiblenetwork.ai/learn/nptel/102106818/298851c179ed) (Video) 47. [Effect-of-NAC-to neturalise-quinone-and-in-vivo-HDX with human RBCs as a model system](https://invisiblenetwork.ai/learn/nptel/102106818/839b902bb541) (Video) 48. [Application of HDX-MS in structural analysis of human hemoglobin in vivo](https://invisiblenetwork.ai/learn/nptel/102106818/6b2c2c71eb65) (Video) 49. [Application of HDX-MS in structural analysis of hemoglobin in vivo-and-desorbed-hemoglobin from nano particle-surface](https://invisiblenetwork.ai/learn/nptel/102106818/ffd6968c9fb0) (Video) 50. [Application-of-HDX-MS-and -CD-spectroscopy-to-monitor-structural changes-of human hemoglobin from nano particle surface](https://invisiblenetwork.ai/learn/nptel/102106818/903c3ec7a304) (Video) 51. [Structure-function correlation of Hb Beckman, a genetic variant of human hemoglobin, using HDX-MS](https://invisiblenetwork.ai/learn/nptel/102106818/3a21d8879fad) (Video) 52. [Structure-function correlation of glutathionyl hemoglobin, a posttranslational variant of human hemoglobin, using HDX-MS](https://invisiblenetwork.ai/learn/nptel/102106818/be2089cbb1f9) (Video) 53. [Imaging mass spectromery of biological tissue samples using MALDI mass spectrometry platform](https://invisiblenetwork.ai/learn/nptel/102106818/5fd08ab9853d) (Video) 54. [Kidney tissue imaging in a nano particle exposed rat model](https://invisiblenetwork.ai/learn/nptel/102106818/6958819e74e7) (Video) 55. [Imaging of kidney tissues of NP exposed rats and imaging of human prostate tissue](https://invisiblenetwork.ai/learn/nptel/102106818/b5ad928d6005) (Video) 56. [Imaging of human prostate cancer tissue using MALDI-MS](https://invisiblenetwork.ai/learn/nptel/102106818/67c251530232) (Video) 57. [Imaging of human prostate cancer tissue using MALDI-MS and analysis of tissue proteomics data](https://invisiblenetwork.ai/learn/nptel/102106818/333aa0ebd8db) (Video) 58. [Desorption Electrospary Ionisation based Mass Spectrometric Imaging of Prostate Cancer Tissue](https://invisiblenetwork.ai/learn/nptel/102106818/39b5e4f42933) (Video) 59. [Fast photochemical oxidation of protein combined to mass spectrometry for prtein footprinting both in vitro and in vivo](https://invisiblenetwork.ai/learn/nptel/102106818/158e1710162e) (Video) 60. [Biosimilar-monitored through mass spectrometric techniques](https://invisiblenetwork.ai/learn/nptel/102106818/c61d9e2106f7) (Video)