# Advanced Fluorescence Microscopy and Image Processing

- Institution: IIT Hyderabad
- Department: Biotechnology
- Teacher: Prof. Gunjan Mehta
- Level: Undergraduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102106553)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102106553)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

## Lectures

1. [Lecture 01_Introduction to Fluorescence Microscopy](https://invisiblenetwork.ai/learn/nptel/102106553/88ccfd6fcd95) (Video)
2. [Lecture 02_Introduction to Optics](https://invisiblenetwork.ai/learn/nptel/102106553/d9639d5068e2) (Video)
3. [Lecture 03_Design of a fluorescence microscope](https://invisiblenetwork.ai/learn/nptel/102106553/1f2afffb5a34) (Video)
4. [Lecture 04_Fluorescent proteins, organic dyes, and protein labelling strategies](https://invisiblenetwork.ai/learn/nptel/102106553/0fb1c53389b6) (Video)
5. [LIVE _Demonstration of the fluorescence microscope, its design and components](https://invisiblenetwork.ai/learn/nptel/102106553/f3c59cdada61) (Video)
6. [Lecture 05_6D imaging, live cell imaging, time lapse imaging](https://invisiblenetwork.ai/learn/nptel/102106553/ef4f5f0fe97f) (Video)
7. [Lecture 06_Quantifying protein dynamics using FRAP, FLIP, FRET](https://invisiblenetwork.ai/learn/nptel/102106553/3c87d4d48c49) (Video)
8. [LIVE_Demonstration for 6D imaging of live cells on a Leica DMi8 inverted fluorescence microscope](https://invisiblenetwork.ai/learn/nptel/102106553/6f218a69cc66) (Video)
9. [Lecture 07_Types of Illumination Epi, TIRF, HILO, light sheet, two photon](https://invisiblenetwork.ai/learn/nptel/102106553/8ecb0e2b9bd6) (Video)
10. [Lecture 08_Confocal microscopy](https://invisiblenetwork.ai/learn/nptel/102106553/f39641f4a8b9) (Video)
11. [LIVE_Demonstration of the Nikon N-SPARC confocal microscope](https://invisiblenetwork.ai/learn/nptel/102106553/52b17eddfc66) (Video)
12. [Lecture 09_Super-Resolution Microscopy (SIM, STED, STORM/PALM)](https://invisiblenetwork.ai/learn/nptel/102106553/6e50b2736ac2) (Video)
13. [Lecture 10_Nanomaterials for fluorescence imaging](https://invisiblenetwork.ai/learn/nptel/102106553/f3ccb49a612d) (Video)
14. [Lecture 11_Immunofluorescence IF and Immunohistochemistry IHC](https://invisiblenetwork.ai/learn/nptel/102106553/4d2ba78765c3) (Video)
15. [Lecture 12_Fluorescence In Situ Hybridization FISH, RNA FISH](https://invisiblenetwork.ai/learn/nptel/102106553/3eb2549fdfee) (Video)
16. [LIVE_Demonstration for immunofluroescence procedure](https://invisiblenetwork.ai/learn/nptel/102106553/3b4efe6932f6) (Video)
17. [Lecture 13_Digital images and camera technologies for microscopy](https://invisiblenetwork.ai/learn/nptel/102106553/9e599d325bb4) (Video)
18. [Lecture 14_CCD, EMCCD, sCMOS Camera](https://invisiblenetwork.ai/learn/nptel/102106553/573e1c1db9dd) (Video)
19. [LIVE_Demonstration for digital images and adjustments](https://invisiblenetwork.ai/learn/nptel/102106553/ace17cdd8436) (Video)
20. [Lecture 15_Basic operations of ImageJ/FIJI, montage preparation](https://invisiblenetwork.ai/learn/nptel/102106553/8182634119e9) (Video)
21. [Lecture 16_Image filtering and object segmentation](https://invisiblenetwork.ai/learn/nptel/102106553/df69c46a308c) (Video)
22. [Lecture 17_Macros, intensity measurement, scale bar, time stamp, colocalization](https://invisiblenetwork.ai/learn/nptel/102106553/851a58e4d807) (Video)
23. [LIVE_Demonstration of ImageJ/Fiji based image processing](https://invisiblenetwork.ai/learn/nptel/102106553/8c4bdc3b1840) (Video)
24. [Lecture 18_Single molecule imaging and tracking](https://invisiblenetwork.ai/learn/nptel/102106553/7d74bfa4770e) (Video)
25. [Lecture 19_Optical tweezers and traction force microscopy](https://invisiblenetwork.ai/learn/nptel/102106553/84ec6c331363) (Video)
26. [LIVE_Demonstration for single-molecule imaging under HILO illumination](https://invisiblenetwork.ai/learn/nptel/102106553/8e2ebf93a2bd) (Video)
27. [Lecture 20_Spatial transcriptomics and proteomics RNAscope, MERFISH, CODEX](https://invisiblenetwork.ai/learn/nptel/102106553/64730a54e1ac) (Video)
28. [Lecture 21_High content imaging](https://invisiblenetwork.ai/learn/nptel/102106553/6633b6391fdc) (Video)
29. [Lecture 22_Designing a fluorescence microscopy experiment](https://invisiblenetwork.ai/learn/nptel/102106553/f4ff1e03efaa) (Video)
30. [Live Session 31-01-2025](https://invisiblenetwork.ai/learn/nptel/102106553/88ccfd6fcd95-2) (Video)
31. [Live Session 07-02-2025](https://invisiblenetwork.ai/learn/nptel/102106553/d9639d5068e2-2) (Video)
32. [Live Session 12-02-2025](https://invisiblenetwork.ai/learn/nptel/102106553/1f2afffb5a34-2) (Video)
33. [Live Session 28-02-2025](https://invisiblenetwork.ai/learn/nptel/102106553/0fb1c53389b6-2) (Video)
34. [Live Session 07-03-2025](https://invisiblenetwork.ai/learn/nptel/102106553/f3c59cdada61-2) (Video)
35. [Live Session 28-03-2025](https://invisiblenetwork.ai/learn/nptel/102106553/ef4f5f0fe97f-2) (Video)
36. [Live Session 11-04-2025](https://invisiblenetwork.ai/learn/nptel/102106553/3c87d4d48c49-2) (Video)