# Genome Editing And Engineering - Institution: IIT Guwahati - Department: Biotechnology - Teacher: Prof. Utpal Bora - Level: Undergraduate - Language: English - Subjects: Biology - Source: [NPTEL](https://nptel.ac.in/courses/102103093) - Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.) - Syllabus: [at the source](https://nptel.ac.in/courses/102103093) - Who can watch: Teens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words) This course is designed for UG & PG students, research scholars and young scientists to introduce them to basics and applications of genome editing and engineering. The course starts with understanding of basic organization and structure of genome. It gives a brief overview on different DNA strand breaks and their repair mechanism. It introduces learners to theoretical basics of genetic engineering and discusses it’s limitations in tackling genetic problems of animals and plants. The key concepts of Genome editing are discussed thoroughly with emphasis on the major genome editing tools ZFN, TALEN and CRISP/Cas9. It discusses the biochemical basis of development of the genome editing tools, their design and their applications in various genetic conditions. It also discusses the scope and prospect of using these technologies in solving major genetic disease of human. The learners will also be acquainted with the ethical concerns associated with applications of genome editing and engineering in germline. Intended audience: UG/PG/PhD/Scientist in industry Prerequisites: 10+2 with Biology and Chemistry ## Lectures 1. [Lec 1: Introduction :Genes and Genome Organization](https://invisiblenetwork.ai/learn/nptel/102103093/02d0ee4f37c5) (YouTube) 2. [Lec 2: History and Basics of Genetic Engineering](https://invisiblenetwork.ai/learn/nptel/102103093/309d4dfdb101) (YouTube) 3. [Lec 3: Advantages and Limitations of Genetic Engineering](https://invisiblenetwork.ai/learn/nptel/102103093/525cacc6692d) (YouTube) 4. [Lec 1: Breakage of Genomic DNA](https://invisiblenetwork.ai/learn/nptel/102103093/c75120acb5cd) (YouTube) 5. [Lec 2: Repair of Genomic DNA](https://invisiblenetwork.ai/learn/nptel/102103093/41fce7b20650) (YouTube) 6. [Lec 1: Homologous and non homologous recombination](https://invisiblenetwork.ai/learn/nptel/102103093/a8f2ee1772ae) (YouTube) 7. [Lec 2: Site specific recombination](https://invisiblenetwork.ai/learn/nptel/102103093/aae31d6a16a1) (YouTube) 8. [Lec 1: Targeted genetic modification - I](https://invisiblenetwork.ai/learn/nptel/102103093/582f0ee18690) (YouTube) 9. [Lec 2: Targeted genetic modification - II](https://invisiblenetwork.ai/learn/nptel/102103093/afcf9803c3d2) (YouTube) 10. [Lec 1: Basics of Zinc Finger Nucleases](https://invisiblenetwork.ai/learn/nptel/102103093/923ab4a100ae) (YouTube) 11. [Lec 2: Design of Zinc Finger Nucleases for genome editing](https://invisiblenetwork.ai/learn/nptel/102103093/dfabdca14666) (YouTube) 12. [Lec 3: Applications of Zinc Finger Nucleases - PART A](https://invisiblenetwork.ai/learn/nptel/102103093/e4816647577c) (YouTube) 13. [Lec 4: Applications of Zinc Finger Nucleases - PART B](https://invisiblenetwork.ai/learn/nptel/102103093/d2b4515d2c32) (YouTube) 14. [Lec 1: Basics of TALEN - PART A](https://invisiblenetwork.ai/learn/nptel/102103093/e88ee5f38929) (YouTube) 15. [Lec 2: Basics of TALEN - PART B](https://invisiblenetwork.ai/learn/nptel/102103093/6a3483112307) (YouTube) 16. [Lec 3: Design of TALEN for genome editing - PART A](https://invisiblenetwork.ai/learn/nptel/102103093/7e199c4b7ada) (YouTube) 17. [Lec 4: Design of TALEN for genome editing - PART B](https://invisiblenetwork.ai/learn/nptel/102103093/4a0876a30b8c) (YouTube) 18. [Lec 5: Application of TALEN - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/047d2a8344c8) (YouTube) 19. [Lec 6: Application of TALEN - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/72652f1d4b5c) (YouTube) 20. [Lec 1: CRISPR system in bacteria - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/8c4f0ad35e99) (YouTube) 21. [Lec 2: CRISPR system in bacteria - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/6441d4b1eee1) (YouTube) 22. [Lec 3: CRISPR/Cas9 in Genome Editing - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/a8693bde7c3b) (YouTube) 23. [Lec 4: CRISPR/Cas9 in Genome Editing - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/848f50bf7a6c) (YouTube) 24. [Lec 5: Applications of CRISPR/Cas9 Part A](https://invisiblenetwork.ai/learn/nptel/102103093/470138ffa3e3) (YouTube) 25. [Lec 6: Applications of CRISPR/Cas9 Part B](https://invisiblenetwork.ai/learn/nptel/102103093/7a379c2afd05) (YouTube) 26. [Lec 7: Computational Resources for CRISPR / Cas - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/3f50405508e3) (YouTube) 27. [Lec 8: Computational Resources for CRISPR / Cas - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/3d370dde541b) (YouTube) 28. [Lec 1: Human cell engineering in diseases : Thalassemia - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/065e6eb50351) (YouTube) 29. [Lec 2: Human cell engineering in diseases : Thalassemia - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/2542396fcda0) (YouTube) 30. [Lec 3: Human cell engineering in diseases : Severe combined immunodeficiency (SCID) - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/cf722ce3fb3f) (YouTube) 31. [Lec 4: Human cell engineering in diseases : Severe combined immunodeficiency (SCID) - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/a0754701619d) (YouTube) 32. [Lec 5: Human cell engineering in diseases : Hemophilia - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/61082eda3506) (YouTube) 33. [Lec 6: Human cell engineering in diseases : Hemophilia - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/5186944202de) (YouTube) 34. [Lec 1: Animal models - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/a8b81d1a8435) (YouTube) 35. [Lec 2: Animal models - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/c3ac92036339) (YouTube) 36. [Lec 3: iPSc models - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/d97b5ac6b073) (YouTube) 37. [Lec 4: iPSc models - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/8c4af921ff3f) (YouTube) 38. [Lec 5: Cancer disease models - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/be7a50c67ec8) (YouTube) 39. [Lec 6: Cancer disease models - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/aa014fdfbf9c) (YouTube) 40. [Lec 1: Engineered immune cells for Cancer therapy (I) - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/0d6efd21d99c) (YouTube) 41. [Lec 2: Engineered immune cells for Cancer therapy (I) - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/3008c3340afd) (YouTube) 42. [Lec 3: Engineered immune cells for Cancer therapy (II) - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/4e0c319220a5) (YouTube) 43. [Lec 4: Engineered immune cells for Cancer therapy (II) - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/cbe64900a6c4) (YouTube) 44. [Lec 1: History and Basics - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/939187f1d444) (YouTube) 45. [Lec 2: History and Basics - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/7894adedaaaa) (YouTube) 46. [Lec 3: Genome editing and personalized therapy](https://invisiblenetwork.ai/learn/nptel/102103093/3278097eda06) (YouTube) 47. [Lec 1: Bioethics and Biosafety - Part A](https://invisiblenetwork.ai/learn/nptel/102103093/27dce90303a8) (YouTube) 48. [Lec 2: Bioethics and Biosafety - Part B](https://invisiblenetwork.ai/learn/nptel/102103093/16aa5d50b7f0) (YouTube) 49. [Lec 3: Regulatory issues in Genome Editing](https://invisiblenetwork.ai/learn/nptel/102103093/8dd2113b9ef6) (YouTube)