# Experimental Biotechnology

- Institution: IIT Guwahati
- Department: Biotechnology
- Teacher: Prof. Vishal Trivedi
- Level: Graduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102103083)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102103083)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. By the end of this course, student will be able to understand: 1. Basics of Good Lab practices. 2. Understanding different analytical techniques and their applications. 3. Specific Scientific questions and their solutions. 4. Designing new experiments.

Intended audience: PG/PhD

Prerequisites: General Biology 10+2

## Lectures

1. [Lec 1: Good Lab Practices (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/4a8b6daf8460) (YouTube)
2. [Lec 2: Good Lab Practices (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/a0e2493a134d) (YouTube)
3. [Lec 3: Operation of Laboratory Instruments (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/c16dd6289a60) (YouTube)
4. [Lec 4: Operation of Laboratory Instruments (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/0b0ffe760e81) (YouTube)
5. [Lec 5: Operation of Laboratory Instruments (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/0c3e8cc8027b) (YouTube)
6. [Lec 6: Solution and Buffer Preparation](https://invisiblenetwork.ai/learn/nptel/102103083/70e7b50c2f53) (YouTube)
7. [Lec 1: Basics of Electrophoresis (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/b5a7b639a3de) (YouTube)
8. [Lec 2: Basics of Electrophoresis (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/f6dfa6b5d175) (YouTube)
9. [Lec 3: Horizontal Gel Electrophoresis](https://invisiblenetwork.ai/learn/nptel/102103083/dc2b82f26300) (YouTube)
10. [Lec 1: Different Varients of Gel Electrophoresis](https://invisiblenetwork.ai/learn/nptel/102103083/5bd307551cdb) (YouTube)
11. [Lec 2: Scientific Questions (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/e5f03d30a30c) (YouTube)
12. [Lec 3: Scientific Questions (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/a7a749da4618) (YouTube)
13. [Lec 4: Scientific Questions (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/b187e2cfcb9a) (YouTube)
14. [Lec 5: Scientific Questions (Part 4)](https://invisiblenetwork.ai/learn/nptel/102103083/361f2c4053e7) (YouTube)
15. [Lec 1: Basics of chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/7a08c865c971) (YouTube)
16. [Lec 2: Basics of chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/fccdf0887cbf) (YouTube)
17. [Lec 3: Ion-exchange chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/e6a9504065e4) (YouTube)
18. [Lec 4: Ion-Exchange Chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/a3ec7ec253c4) (YouTube)
19. [Lec 5: Hydrophobic Interaction Chromatography](https://invisiblenetwork.ai/learn/nptel/102103083/66c391a19f3a) (YouTube)
20. [Lec 1: Gel filtration chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/d6cf4e7993bd) (YouTube)
21. [Lec 2: Gel Filtration Chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/6931e2d357a9) (YouTube)
22. [Lec 3: Gel Filtration Chromatography (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/4772dbe61631) (YouTube)
23. [Lec 1: Affinity chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/f0c3e52d8c4e) (YouTube)
24. [Lec 2: Affinity Chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/8633cb1094e8) (YouTube)
25. [Lec 3: Affinity Chromatography (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/10b59c232969) (YouTube)
26. [Lec 4: Affinity Chromatography (Part 4)](https://invisiblenetwork.ai/learn/nptel/102103083/dfcfdd767649) (YouTube)
27. [Lec 1: Antibody generation](https://invisiblenetwork.ai/learn/nptel/102103083/aacf3636b5a9) (YouTube)
28. [Lec 2: Antibody-Antigen Interaction (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/f82ac25dffdc) (YouTube)
29. [Lec 3: Immunoassay](https://invisiblenetwork.ai/learn/nptel/102103083/35f515ebd248) (YouTube)
30. [Lec 4: Antibody-Antigen Interaction (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/8ac07356cf8e) (YouTube)
31. [Lec 5: Antibody-Antigen Interaction (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/274f00bca67d) (YouTube)
32. [Lec 1: Cell Culture Medium](https://invisiblenetwork.ai/learn/nptel/102103083/08162fc1da18) (YouTube)
33. [Lec 2: Cell Fractionation](https://invisiblenetwork.ai/learn/nptel/102103083/186dd6607a59) (YouTube)
34. [Lec 1: Microscopy (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/c23c34a2dece) (YouTube)
35. [Lec 2: Microscopy (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/18f1a4609c6b) (YouTube)
36. [Lec 3: Cell Biology Experiments](https://invisiblenetwork.ai/learn/nptel/102103083/983585669e8c) (YouTube)
37. [Lec 4: Flow cytometry](https://invisiblenetwork.ai/learn/nptel/102103083/107278ecb534) (YouTube)
38. [Lec 1: Polymerase chain reaction (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103083/ac7066d6e84d) (YouTube)
39. [Lec 2: Polymerase chain reaction (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103083/5af4ea497bd3) (YouTube)
40. [Lec 3: Polymerase chain reaction (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103083/9f646e12ff2b) (YouTube)
41. [Lec 4: Polymerase chain reaction (Part 4)](https://invisiblenetwork.ai/learn/nptel/102103083/d5c6dbddd17b) (YouTube)
42. [Lec 1: Sequencing Techniques](https://invisiblenetwork.ai/learn/nptel/102103083/a97f29b6c6c9) (YouTube)
43. [Lec 2: Blotting Techniques (Part-1)](https://invisiblenetwork.ai/learn/nptel/102103083/bea959674f81) (YouTube)
44. [Lec 3: Blotting Techniques (Part-2)](https://invisiblenetwork.ai/learn/nptel/102103083/2271d045d77d) (YouTube)
45. [Lec 1: Designing Experiments](https://invisiblenetwork.ai/learn/nptel/102103083/ff8749b29cfd) (YouTube)