# Genetic Engineering: Theory And Application

- Institution: IIT Guwahati
- Department: Biotechnology
- Teacher: Prof. Vishal Trivedi
- Level: Undergraduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102103074)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102103074)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

In the current MOOCs course I have put effort to briefly discuss about biotechnology, its scope and impact on human life with several customized products. The Development of technology and generation of product has multiple steps and understanding these steps are being covered in this course with a discussion of biotechnology application at the end. By the end of this course, student will be able to understand following aspects of biotechnology:

1. Basic metabolic pathways and their regulation.

2. Microbial growth kinetics with an emphasis on fermentation

3. Basic molecular biology tools used in biotechnology.

4. Basic methodology for product recovery and analysis.

INTENDED AUDIENCE : UG/PG/PhD/Scientist in industry

PREREQUISITES : General Biology 10+2

INDUSTRY SUPPORT : Biocon https://www.biocon.com/Jubilant Life Sciences www.jubl.com/ ,Shantha Biotechnics Ltd,Panacea Biotec,Other companies related to biotechnology

## Lectures

1. [Lec 1: Cellular Structure (Part I)](https://invisiblenetwork.ai/learn/nptel/102103074/7c696731f568) (YouTube)
2. [Lec 2: Cellular Structure (Part II)](https://invisiblenetwork.ai/learn/nptel/102103074/d818c14e8f30) (YouTube)
3. [Lec 3: Cellular Structure (Part III)](https://invisiblenetwork.ai/learn/nptel/102103074/db76a96d6d67) (YouTube)
4. [Lec 1: Metabolic Reactions in Biological System](https://invisiblenetwork.ai/learn/nptel/102103074/1fa17e9d6ef1) (YouTube)
5. [Lec 2: Growth Media For Different Expression System](https://invisiblenetwork.ai/learn/nptel/102103074/2e381da12d53) (YouTube)
6. [Lec 3: Microbial Growth Kinetics](https://invisiblenetwork.ai/learn/nptel/102103074/f3a2189b9319) (YouTube)
7. [Lec 1: Isolation of a Gene Fragment (Part I)](https://invisiblenetwork.ai/learn/nptel/102103074/372c08bb4906) (YouTube)
8. [Lec 2: Isolation of a Gene Fragment (Part II)](https://invisiblenetwork.ai/learn/nptel/102103074/d733c3423cf0) (YouTube)
9. [Lec 3: Isolation of a Gene Fragment (Part III)](https://invisiblenetwork.ai/learn/nptel/102103074/b9084a6dbe3e) (YouTube)
10. [Lec 4: Polymerase Chain Reaction](https://invisiblenetwork.ai/learn/nptel/102103074/3bc693fcb722) (YouTube)
11. [Lec 1: Molecular Tools for Cloning](https://invisiblenetwork.ai/learn/nptel/102103074/d6395c5a2db6) (YouTube)
12. [Lec 2: Cloning Vectors I](https://invisiblenetwork.ai/learn/nptel/102103074/86fcc5815560) (YouTube)
13. [Lec 3: Cloning Vectors II](https://invisiblenetwork.ai/learn/nptel/102103074/bc7718201ded) (YouTube)
14. [Lec 1: DNA Delivery In Host (Part I)](https://invisiblenetwork.ai/learn/nptel/102103074/381b6d3d1beb) (YouTube)
15. [Lec 2: DNA Delivery In Host (Part II)](https://invisiblenetwork.ai/learn/nptel/102103074/01588788a001) (YouTube)
16. [Lec 3: Screening of Recombinant Clones](https://invisiblenetwork.ai/learn/nptel/102103074/f0199a4ead8e) (YouTube)
17. [Lec 1: Protein Production in Host (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/3447cc8429a2) (YouTube)
18. [Lec 2: Protein Production in Host (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/a4a1eff04859) (YouTube)
19. [Lec 3: Protein Production in Host (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103074/db85f5f54fbe) (YouTube)
20. [Lec 1: Product Recovery from Host Cells](https://invisiblenetwork.ai/learn/nptel/102103074/22ac86e40ac3) (YouTube)
21. [Lec 2: Basics of Chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/62442576f93b) (YouTube)
22. [Lec 3: Basics of Chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/59366ec0af2f) (YouTube)
23. [Lec 4: Ion-exchange Chromatography](https://invisiblenetwork.ai/learn/nptel/102103074/be9b17da087e) (YouTube)
24. [Lec 5: Hydrophobic Interaction chromatography](https://invisiblenetwork.ai/learn/nptel/102103074/00516e076620) (YouTube)
25. [Lec 1: Gel Filtration chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/13993f7e0a4b) (YouTube)
26. [Lec 2: Gel Filtration chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/a58f6fc5f412) (YouTube)
27. [Lec 3: Affinity Chromatography (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/85913dc40b7f) (YouTube)
28. [Lec 4: Affinity Chromatography (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/28c202836755) (YouTube)
29. [Lec 5: Affinity Chromatography (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103074/5f6880bb3bbe) (YouTube)
30. [Lec 6: Affinity Chromatography (Part 4)](https://invisiblenetwork.ai/learn/nptel/102103074/6c6e1396ddf5) (YouTube)
31. [Lec 1: Electrophoresis (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/d90afb949de6) (YouTube)
32. [Lec 2: Electrophoresis (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/cb5ba1f9d237) (YouTube)
33. [Lec 3: Electrophoresis (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103074/a455ecc4ed82) (YouTube)
34. [Lec 1: Protein Sequencing](https://invisiblenetwork.ai/learn/nptel/102103074/9fa2dbbc9f31) (YouTube)
35. [Lec 2: Spectroscopy (Part I)](https://invisiblenetwork.ai/learn/nptel/102103074/4f9b862f8fae) (YouTube)
36. [Lec 3: Spectroscopy (Part II)](https://invisiblenetwork.ai/learn/nptel/102103074/b8fea6b8ed07) (YouTube)
37. [Lec1: Biotechnology Applications (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/73b0b3382698) (YouTube)
38. [Lec2: Biotechnology Applications (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/b769393d27ce) (YouTube)
39. [Lec3: Biotechnology Applications (Part 3)](https://invisiblenetwork.ai/learn/nptel/102103074/d0d962982c54) (YouTube)
40. [Lec1 : Summary and conclusions (Part 1)](https://invisiblenetwork.ai/learn/nptel/102103074/c709c3dbc52b) (YouTube)
41. [Lec2: Summary and conclusions (Part 2)](https://invisiblenetwork.ai/learn/nptel/102103074/22bb1a7145bb) (YouTube)
