# Mass spectrometry based proteomics

- Institution: IIT Bombay
- Department: Biotechnology
- Teacher: Prof. Sanjeeva Srivastava
- Level: Undergraduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102101050)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102101050)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

## Lectures

1. [Introductory lecture](https://invisiblenetwork.ai/learn/nptel/102101050/3daeb77a2be8) (Video)
2. [Lecture 1: Introduction to proteomics](https://invisiblenetwork.ai/learn/nptel/102101050/43572ebe4cc2) (Video)
3. [Lecture 2: Proteomics and sample preparation](https://invisiblenetwork.ai/learn/nptel/102101050/63501428a642) (Video)
4. [Lecture 3: Bacterial protein extraction](https://invisiblenetwork.ai/learn/nptel/102101050/9795fcbb6aad) (Video)
5. [Lecture 4: In-gel digestion](https://invisiblenetwork.ai/learn/nptel/102101050/57641f26aeae) (Video)
6. [Lecture 5: Fundamentals of mass spectrometry](https://invisiblenetwork.ai/learn/nptel/102101050/ffa23f871d42) (Video)
7. [Lab session 1.1 : Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR](https://invisiblenetwork.ai/learn/nptel/102101050/608d6ab0454b) (Video)
8. [Lab Session 1.2: Demonstration of Q-TOF MS technology](https://invisiblenetwork.ai/learn/nptel/102101050/7684c8eb4a10) (Video)
9. [Lecture 6: Chromatography technologies](https://invisiblenetwork.ai/learn/nptel/102101050/a4421a82d744) (Video)
10. [Lecture 7: Liquid chromatography](https://invisiblenetwork.ai/learn/nptel/102101050/725e91ebf13d) (Video)
11. [Lecture 8: Mass spectrometry: Ionization sources](https://invisiblenetwork.ai/learn/nptel/102101050/5537b42bcbd3) (Video)
12. [Lecture 9: Mass spectrometry: Mass analyzers](https://invisiblenetwork.ai/learn/nptel/102101050/8a26ceae2e96) (Video)
13. [Lecture 10: MALDI sample preparation and analysis](https://invisiblenetwork.ai/learn/nptel/102101050/b10b72bc155b) (Video)
14. [Lab session 2.1: Global proteomics - experimental design and sample preparation](https://invisiblenetwork.ai/learn/nptel/102101050/bb6ff3aa6950) (Video)
15. [Lab session 2.2: Global proteomics - instrumentation and data acquisition](https://invisiblenetwork.ai/learn/nptel/102101050/0318660c6442) (Video)
16. [Lecture 11: Introduction to quantitative proteomics](https://invisiblenetwork.ai/learn/nptel/102101050/9fc9ea00e4ba) (Video)
17. [Lecture 12: Hybrid mass spectrometry configurations](https://invisiblenetwork.ai/learn/nptel/102101050/3b27607b0f58) (Video)
18. [Lecture 13: SILAC: In Vivo labeling](https://invisiblenetwork.ai/learn/nptel/102101050/88c564d9b557) (Video)
19. [Lecture 14: iTRAQ: In Vitro labeling](https://invisiblenetwork.ai/learn/nptel/102101050/4e4266360051) (Video)
20. [Lecture 15: TMT: In vitro labeling](https://invisiblenetwork.ai/learn/nptel/102101050/e7c2c702c7f0) (Video)
21. [Lab session 3.1: Targeted proteomics - experimental design](https://invisiblenetwork.ai/learn/nptel/102101050/7b23aa8dce11) (Video)
22. [Lab session 3.2: Targeted proteomics - sample preparation, data acquisition and data analysis using skyline](https://invisiblenetwork.ai/learn/nptel/102101050/26bab29feedc) (Video)
23. [Lecture 16: Quantitative proteomics data analysis](https://invisiblenetwork.ai/learn/nptel/102101050/a6874d242b41) (Video)
24. [Lecture 17: Proteomics and Systems biology I](https://invisiblenetwork.ai/learn/nptel/102101050/0ed00c24b511) (Video)
25. [Lecture 18: Proteomics & Systems biology II](https://invisiblenetwork.ai/learn/nptel/102101050/6ec88b0bf8d9) (Video)
26. [Lecture 19: Proteomics applications](https://invisiblenetwork.ai/learn/nptel/102101050/6edc9f78ea5b) (Video)
27. [Lecture 20: Advances and challenges in proteomics](https://invisiblenetwork.ai/learn/nptel/102101050/af98e9e14cb2) (Video)
28. [Lab session 4.1: Protein-protein interaction using label-free biosensors](https://invisiblenetwork.ai/learn/nptel/102101050/1f566400c2ee) (Video)