# Proteins and Gel-Based Proteomics

- Institution: IIT Bombay
- Department: Biotechnology
- Teacher: Prof. Sanjeeva Srivastava
- Level: Undergraduate
- Language: English
- Subjects: Biology
- Source: [NPTEL](https://nptel.ac.in/courses/102101049)
- Licence: [CC BY-NC-SA](https://nptel.ac.in/faq) (Non-commercial use only.)
- Syllabus: [at the source](https://nptel.ac.in/courses/102101049)
- Who can watch: Tweens profiles and older (sources give no ratings; placed by Invisible Network from the source's own words)

This course introduces to the basic biology of proteins and gel-based proteomics. To obtain better understanding of cellular processes & regulation, there has been an increasing interest in studying proteomics. Proteomics techniques aim to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. These proteomic techniques typically aim to elucidate the expression, interaction, and cellular function of proteins.

The course will provide the basic understanding of amino acids and proteins. It would also provide detailed information on gel-based proteomic techniques such as SDS-PAGE and 2-DE. Some of the limitation of 2-DE has been overcome by advanced Difference gel electrophoresis (DIGE) technique, which will be discussed in this course. Protein identification using MALDI-TOF/TOF mass spectrometry will also be discussed. 2-DE in combination with mass spectrometry has become very powerful tool for proteomics applications and students can learn these tools by attending this course.

INTENDED AUDIENCE

Core course

All UG and PG students can attend this course.

It would be applied to B.Sc., M.Sc. and Ph.D

PRE-REQUISITES

B.Sc. Or M.Sc.

The target audiences of this course are required to have a basic biology and biochemistry background.

## Lectures

1. [L1. Introduction to amino acids](https://invisiblenetwork.ai/learn/nptel/102101049/ce21bbfeba83) (YouTube)
2. [L2. Introduction to proteins](https://invisiblenetwork.ai/learn/nptel/102101049/a9a544903a99) (YouTube)
3. [L3. Protein folding & misfolding](https://invisiblenetwork.ai/learn/nptel/102101049/89146c2a1705) (YouTube)
4. [L4. Protein purification techniques](https://invisiblenetwork.ai/learn/nptel/102101049/5c709c115a30) (YouTube)
5. [L5. Introduction to proteomics](https://invisiblenetwork.ai/learn/nptel/102101049/908ed327b7ab) (YouTube)
6. [Lab Session-1.1: Demonstration of protein-protein interactions using label-free biosensors](https://invisiblenetwork.ai/learn/nptel/102101049/4f7348156efe) (YouTube)
7. [L6. Systems biology and proteomics](https://invisiblenetwork.ai/learn/nptel/102101049/565ce6a8959c) (YouTube)
8. [L7. Sample preparation and pre-analytical factors](https://invisiblenetwork.ai/learn/nptel/102101049/7321c000921e) (YouTube)
9. [L8. Sample preparation: Pre-analytical factors (contd.)](https://invisiblenetwork.ai/learn/nptel/102101049/4ced08969027) (YouTube)
10. [L9. Sample preparation: Protein extraction and quantification](https://invisiblenetwork.ai/learn/nptel/102101049/bfb7a70533bb) (YouTube)
11. [L10. One-dimensional electrophoresis](https://invisiblenetwork.ai/learn/nptel/102101049/2fc2bc04fa2f) (YouTube)
12. [Lab session-2.1: Sample preparation: Tissue sample preservation technology](https://invisiblenetwork.ai/learn/nptel/102101049/a44d2cc29276) (YouTube)
13. [Lab session-2.2: Protein/peptide pre-fractionation using Off-gel fractionator](https://invisiblenetwork.ai/learn/nptel/102101049/ef885e5ca228) (YouTube)
14. [L11. 2-DE: Rehydration, IEF & Equilibration](https://invisiblenetwork.ai/learn/nptel/102101049/670ba976a02a) (YouTube)
15. [L12. 2-DE: Second dimension, staining & destaining](https://invisiblenetwork.ai/learn/nptel/102101049/577e236c743e) (YouTube)
16. [L13. 2-DE: Gel analysis](https://invisiblenetwork.ai/learn/nptel/102101049/7e95762b7241) (YouTube)
17. [L14. 2-DE: Applications](https://invisiblenetwork.ai/learn/nptel/102101049/4a3115d63624) (YouTube)
18. [L15. 2-DE: Applications (contd.) & Challenges](https://invisiblenetwork.ai/learn/nptel/102101049/9e4c3af8263b) (YouTube)
19. [Lab session-3.1: Demonstration of gel analysis using IQTL software](https://invisiblenetwork.ai/learn/nptel/102101049/9ba1cbdba173) (YouTube)
20. [L16. 2D-DIGE: Basics](https://invisiblenetwork.ai/learn/nptel/102101049/d487bfaf2ec7) (YouTube)
21. [L17. 2D-DIGE: Data analysis](https://invisiblenetwork.ai/learn/nptel/102101049/d19dc20ef53c) (YouTube)
22. [L18. 2D-DIGE: Applications](https://invisiblenetwork.ai/learn/nptel/102101049/d0ed7ec5072f) (YouTube)
23. [L19. Protein identification using MALDI-TOF/TOF](https://invisiblenetwork.ai/learn/nptel/102101049/924847628271) (YouTube)
24. [L20. Proteomics experiment data analysis & challenges](https://invisiblenetwork.ai/learn/nptel/102101049/14539ceb7cf8) (YouTube)
25. [Lab session-4.1: Demonstration of Q-TOF MS technology](https://invisiblenetwork.ai/learn/nptel/102101049/a8397571978d) (YouTube)