# STD Depth Coded Stack Phallodin Stained Actin Filaments

- By: Howard Vindin
- Date: 2014-07-01
- Classification: Photograph
- Museum: [Wikimedia Commons](https://commons.wikimedia.org/wiki/File:STD_Depth_Coded_Stack_Phallodin_Stained_Actin_Filaments.png)
- Rights: [CC BY-SA 4.0](https://creativecommons.org/licenses/by-sa/4.0)
- Picture: https://invisiblenetwork.ai/thumbs/commons/thumb/6/69/STD_Depth_Coded_Stack_Phallodin_Stained_Actin_Filaments.png/1920px-STD_Depth_Coded_Stack_Phallodin_Stained_Actin_Filaments.png
- Who can see it: Teens profiles and older (museums give no ratings; placed by Invisible Network from the museum's own words)

A merged stack of confocal images showing actin filaments within a cell. The image has been colour coded in the z axis to show in a 2D image which heights filaments can be found at within cells.

Dimensions

XY: 4096x4096 px (167.92x167.92 µm)

Z: 103 slices (8.56 µm) (merged into a single slice)

Scanner Settings

ScanMode: xyz

Pinhole \[m\]: 95.5 µm

Pinhole \[airy\]: 1.00

Total Size-Width: 167.9 µm

Total Size-Height: 167.9 µm

Total Size-Depth: 8.6 µm

StepSize: 0.08 µm

Voxel-Width: 41.0 nm (pixel size)

Voxel-Height: 41.0 nm (pixel size)

Voxel-Depth: 83.9 nm

Voxel-Volume: 141035.9 nm³

Zoom: 1.5

Objective: HCX PL APO CS 63.0x1.40 OIL UV

Numerical aperture: 1.40

